Estimation of Human Flavin-containing Monooxygenases Activity ( FMO1 ) in the Baculovirus Expression Vector System by using S-oxidation of Methimazole
(주)코리아스칼라
- 최초 등록일
- 2023.04.05
- 최종 저작일
- 1999.12
- 7페이지/ 어도비 PDF
- 가격 4,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 14권 / 4호
ㆍ저자명 : Kim,Young MI
영어 초록
The flavin-containing monooxygenases (FMOs) (EC 1.14.13.8) are NADPH-dependent flavoenzymes that catalyze oxidation of soft nucleophilic heteroatom centers in a range of structurally diverse compounds including foods, drugs, pesticides, and other xenobiotics. In humans, FMO1 appears to be the predominant form expressed in human fetal liver. cDNA-expressed human FMO and human liver microsomal FMO have been observed to N- and S-oxygenate nucleophilic nitrogen- and sulfur-containing drugs and chemicals, respectively. In the present study, FMOl can be expressed in the baculovirus expression vector system at level of 2.68 nmol FMO1/㎎ of membrane protein. This isoform was examined for its capacity to metabolize methimazole to its S-oxide using thiocholine assay. Kinetic studies of its S-oxide by recombinant human FMO1 result in K_m of 7.66 μM and V_(max) of 17.79 nmol/min/㎎ protein.
참고 자료
없음
"한국식품위생안전성학회지"의 다른 논문
더보기 (5/10)