미니돼지 정액 보존에 있어서 해양성 광천수의 영향

서지정보

ㆍ발행기관 : 강원대학교 동물자원공동연구소 ㆍ수록지정보 : 동물자원연구 / 21권 / 1호
ㆍ저자명 : 이상희, 이재흥, 정희태, 양부근, 김효미, 이샛별, 권순성, 최선강, 심재만, 김정득, 박춘근

목차

ABSTRACT I. 서론 II. 재료 및 방법 1. 시료 준비 및 시약 2. 해양성 광천수의 삼투압 측정 3. 정액 채취 및 운반 4. 정액의 보존 5. 정액의 성상평가 III. 결과 및 고찰 1. 해양성 광천수의 삼투압 측정 2. 미니돼지 정액 보존 시 해양성 광천수가 정자의 생존율에 미치는 효과 3. 첨체반응과 수정능 획득 정자 4. HOST 양성반응율 5. 정자의 기형율 평가 IV. 요약 사사 V. 인용문헌

영어 초록

The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.

참고 자료

없음