The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one..
Miniature pig sperm cryopreservation is continually researched in biotechnology for breed conservation ... Miniature pig sperm was separated from semen by washing with modified- Modena B (mMB) extender. ... In this study, we suggest that trehalose is effective in reducing freezing damage to miniature pig sperm
In conclusion, supplement of NA in liquid semen of miniature pig can improve and maintain semen quality ... pig. ... study was to investigate the effect of nicotinic acid (NA) on the characteristics in fresh semen of miniature
The percentage of F-patterned spermatozoa in miniature pig was significantly (p<0.05) lower, while ... Abnormality of spermatozoa in miniature pig was significantly (p<0.05) higher than that in Duroc at ... In conclusion, this study suggest that suitable freezing method for miniature pig semen is required for
pig breeds because the size of the organs of the miniature pig is similar to that of humans. ... The miniature pig is considered to be a better organ donor breed for xenotransplantation than other ... For this, we used the miniature pig ovary model, annealing control primer‐based reverse transcription
The present study was conducted to assess sperm characteristics in miniature-pig. ... The rates of AR pattern were higher in miniature-pig than in Duroc and Yorkshire. ... During 3 days of storage, sperm viability did not differ among miniature-pig, Duroc and Yorkshire.
When miniature pig (m) and landrace pig (p) were used as donor cells, there were no differences in cleavage ... The present results suggest that the NT between donor cells derived from miniature pig and recipient ... pig.
In addition, we examined the acrosome reaction of sperm in miniature pigs. ... storage in pigs. ... effects of L-carnitine (LC) and nicotinic acid (NA) on sperm viability during liquid storage at 18℃ in miniature
To avoid hyperacute rejection of xenografts, α1,3-galactosyltransferase knock-out (GalT KO) pigs have ... examined whether Sia-containing glycoconjugates are important as an immunogenic non-Gal epitope in the pig ... Taken together, these finding suggested that the deficiency of GalT gene in pigs resulted in increased
The purpose of this study was to assess sperm quality during in vitro storage of miniature-pig semen ... Freshly ejaculated miniature-pig's semen was diluted with same volumes of Beltsville Thawing Solution ... to determine which extender should be used and how extender can be diluted for in vitro storage of miniature-pig
Thus, although female and male cloned miniature pigs may be physiologically similar to normal animals ... The above variation must be taken into account before considering cloned female or male miniature pigs ... pigs.
CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated ... Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared ... pig for production of bio-organ.
Transgene construct including hCD59 and ILI-18BP was introduced into miniature pig fetal fibroblasts. ... Further study on the transfer of these embryos to surrogates may produce transgenic clone miniature pigs ... present study was designed to produce nuclear transfer embryos, prior to the production of transgenic pigs
Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs ... However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are ... pigs.
To detect the regulatory region of ICAM2 promoter, we cloned 3.7 kb size mini-pig ICAM2 promoter. ... However, pig to human transplantation as known as xeno-transplantation has major problem as immunorejection ... Recently, the solutions of pig to human xenotransplantation are commonly mentioned as having a genetically
Somatic cell nuclear transfer (SCNT) for miniature pig has been developed for xenotransplantation and ... To optimize the surrogate mother condition for improvement of cloned miniature pigs efficiency, we investigated ... We obtained 19 cloned miniature piglets from seven surrogate gilts and five piglets are living healthy
genes were differentially expressed in between each cloned pigs. ... Each group was consist of cloned pigs derived from same cell line (n=3 and 4, respectively). ... All SLA sequences and protein translations were identical among the each pig in the same group.