Green synthesis of fluorescent carbon dots from carrot juice for in vitro cellular imaging
(주)학지사
- 최초 등록일
- 2017.06.14
- 최종 저작일
- 2017.01
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서지정보
ㆍ발행기관 : 한국탄소학회
ㆍ수록지정보 : Carbon Letters / 21권
ㆍ저자명 : Yang Liu, Yanan Liu, Mira Park, Soo-Jin Park, Yifan Zhang, Md Rashedunnabi Akanda, Byung-Yong Park, Hak Yong Kim
목차
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
영어 초록
We report the use of carrot, a new and inexpensive biomaterial source, for preparing high
quality carbon dots (CDs) instead of semi-conductive quantum dots for bioimaging application.
The as-derived CDs possessing down and up-conversion photoluminescence features
were obtained from carrot juice by commonly used hydrothermal treatment. The corresponding
physiochemical and optical properties were investigated by electron microscopy, fluorescent
spectrometry, and other spectroscopic methods. The surfaces of obtained CDs were
highly covered with hydroxyl groups and nitrogen groups without further modification. The
quantum yield of as-obtained CDs was as high as 5.16%. The cell viability of HaCaT cells
against a purified CD aqueous solution was higher than 85% even at higher concentration
(700 μg mL−1) after 24 h incubation. Finally, CD cultured cells exhibited distinguished blue,
green, and red colors, respectively, during in vitro imaging when excited by three wavelength
lasers under a confocal microscope. Offering excellent optical properties, biocompatibility,
low cytotoxicity, and good cellular imaging capability, the carrot juice derived CDs
are a promising candidate for biomedical applications.
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