초급속 동결보존한 체외수정란 유래의 형질전환 마우스 생산효율성 검토
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 30권 / 3호
ㆍ저자명 : 김현, 최창용, 성환후
ㆍ저자명 : 김현, 최창용, 성환후
목차
서 론재료 및 방법
1. 체외수정
2. 도입유전자의 구조와 조정
3. 수정란의 동결 및 융해
4. 마우스 전핵기란에 도입유전자의 미세주입과 배이식
5. 서던블럿 분석
6. Tg 마우스의 발현해석
7. 통계분석
결 과
고 찰
요 약
REFERENCES
영어 초록
Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection. In this study, we used in vitro fertilized mouse eggs, followed by ultrarapid freezing to establish a simple procedure for production of Tg mice. We produced in vitro fertilized mouse eggs and cryopreserved them by ultrarapid freezing method. A total of 139 cryopreserved-thawed pronuclear eggs, of which 101 (72.6%) were survived following microinjection of chicken b-actin promoter-driven firefly improved luciferase cDNA (β-act/luc+) and were transferred into 5 recipients. All recipients became pregnant and gave birth to a total of 15 (14.8%) pups. As a control, same DNA construction (β- act/luc+) was also injected into 450 in vitro fertilized eggs, of which 338 (75.1%) were survived and then were transferred into 14 recipients. Eleven (78%) mice became pregnant and littered a total of 54 (19.1%) pups. Southern blotting analysis of Tg mice indicated that one (1/15, 6.6%) and three (3/54, 5.5%) transgenic mice were production from cryopreserved and in vitro fertilized eggs, respectively. All Tg mice produced from both eggs showed the expression of improved luciferase gene. These results indicated that efficiency of produced of Tg mice from cryopreserved eggs was comparable to that from in vitro fertilized eggs. Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved by ultrarapid freezing is an easy and conveniently method for production of Tg mice.참고 자료
없음"Journal of Embryo Transfer"의 다른 논문
- 침투성 동결보호제가 포유류 초기배자의 생존성에 미치는 영향6페이지
- Unilateral Hydroureteronephrosis caused by Adhesion of ..3페이지
- 자궁내막염 혹은 자궁축농증 발병 젖소의 생식기 내 분포하는 세균 동정 증례4페이지
- hPDX1 유전자의 삽입에 의한 직접 췌도세포 분화7페이지
- 돼지 단위생식란의 세포사멸 유전자 발현 양상에 관한 연구10페이지
- Comparisons of Development Potential in Bovine SCNT Emb..9페이지
- Prolonged Expression of Exogenous GFP Gene in the Porci..4페이지
- 체세포 핵치환 기술을 이용한 hGCSF와 GFP 유전자 동시발현 형질전환 소 배아 생산6페이지
- Hoechst 33258 Staining을 이용한 웅성 생쥐 성세포의 간편 분류6페이지
- 골다공증 모델의 뼈 재생기에 있어 셀레늄(Selenium)의 방사선 보호작용6페이지