일반생물실험 랩 영어 레포트 4. 겔 전기영동 (Gel electrophoresis)

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"일반생물실험 랩 영어 레포트 4. 겔 전기영동 (Gel electrophoresis)"에 대한 내용입니다.

목차

1. Introduction
2. Materials
3. Methods
4. Results
5. Discussion
6. References

본문내용

Introduction
Gel electrophoresis is used to separate, identify, and purify DNA fragments. The agarose gels can be poured in a variety of shapes and sizes, depending on how many wells are needed to run all the samples, and in porosities by controlling the density of the gel through different concentrations. These gels when completely hardened, are placed in a pre-prepared buffer including ethidium bromide (EtBr) or SYBR Gold, fluorescent intercalating dyes which helps determine DNA within the agarose gel by staining the molecules.

참고 자료

Borst, P. (Nov 2005). "Ethidium DNA agarose gel electrophoresis: how it started". IUBMB Life. 57 (11): 745–747.
Sigmon, J.; Larcom, L. L. (Oct 1996). "The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis". Electrophoresis. 17 (10): 1524–1527