일반생물실험 랩 영어 레포트 1. 형질전환 (Transformation)

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"일반생물실험 랩 영어 레포트 1. 형질전환 (Transformation)"에 대한 내용입니다.

목차

1. Introduction
2. Materials
3. Methods
4. Results
5. Discussion
6. References

본문내용

Transformation is a versatile genetic engineering technique that allows insertion of foreign DNA to a competent cell that can amplify the gene. In order to achieve efficient transformation, most importantly, there needs to be a competent cell. To increase the competency of the cell, CaCl2 is added to allow DNA to permeate the cell membrane by destabilizing the phosphate backbone of the membrane. While the mechanism is not known, heat shock at 42℃ is done also to help increase permeability.

참고 자료

Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) “Improved M13 phage cloning vectors and host strains; Nucleotide sequences of the M13mp18 and pUC19 vectors”. Gene 33 (1): 103-119