기수산 물벼룩에서 수온과 polystyrene beads의 복합 독성

서지정보

ㆍ발행기관 : 한국환경생물학회 ㆍ수록지정보 : 환경생물 / 41권 / 4호
ㆍ저자명 : 이윤하, 박종석, 박채린, 조상현, 유제원, 이영미

목차

1. 서 론
2. 재료 및 방법
2.1. 시험생물
2.2. 시험용액 제조
2.3. 노출시험
2.4. 생식지표 및 개체군 성장률 관찰
2.5. Total RNA 추출 및 cDNA 합성
2.6. Quantitative real-time polymerase chainreaction (qRT-PCR)
2.7. 통계분석
3. 결과 및 고찰
3.1. 온도와 PS beads의 만성 노출이 생식지표와개체군 성장률에 미치는 영향
3.2. 온도 및 PS beads의 급성 노출이 항산화 효소,스트레스, 생식 경로 관련 유전자 발현에미치는 영향
3.3. 생식지표, 성장률, 유전자 발현과 온도 및PS beads 크기 사이의 상호작용 효과
4. 결 론
적 요
CRediT authorship contribution statement
Declaration of Competing Interest
REFERENCES

영어 초록

Microplastics and nanoplastics (NMPs) are considered one of hazardous contaminants in marine ecosystems due to their toxic effects, such as reproduction disorder and oxidative stress, on marine organisms. Although water temperature is rising due to global climate change, little information on the toxicological interaction between NMPs and temperature is available. Therefore, in this study, we confirmed the toxicity of NMPs (polystyrene [PS] beads; 0.05- and 6-μm) on brackish water fleas (Diaphanosoma celebensis) depending on increased temperature (30°C and 35°C) at individual and molecular levels. In the chronic toxicity test, the group exposed to high temperatures showed an earlier first reproduction time compared to the normal temperatures group, but it was delayed by co-exposure to NMPs at 35°C. Notably, the total reproduction decreased significantly only after 0.05-μm PS beads exposure at 30°C. Interaction analysis showed that first reproduction time, modulation of the antioxidantrelated gene (GSTS1), heat shock gene (Hsp70), and ecdysteroid pathway-related genes (EcR_A, EcR_B, and CYP314A1) were closely related to temperature and PS beads size. These results indicate that microplastics have size-dependent toxicity, and their toxicity can be enhanced at high temperatures. In addition, higher temperatures and PS beads exposure may have negative effects on reproduction. This study suggests that various factors such as water temperature should be considered when evaluating the toxicity of microplastics in marine ecosystems, and provides an understanding of the complex toxic interaction between water temperature and microplastics for marine zooplankton.

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