덱사메타손으로 유도된 근위축 C2C12 모델에서 효소처리스테비아의 보호 효과

서지정보

ㆍ발행기관 : 한국식품위생안전성학회 ㆍ수록지정보 : 한국식품위생안전성학회지 / 38권 / 2호
ㆍ저자명 : 오건, 최선일, 한웅호, 문효, 이세정, 임지현, 이호성, 정형동, 라문진, 권민희, 이옥환

목차

Materials and Methods
실험재료 및 시약
C2C12 세포 배양 및 분화
XTT assay를 이용한 세포 생존율 측정
Jenner-Giemsa 염색 및 fusion index 측정
Myotube diameter 측정
Western blot 분석
통계처리
Results and Discussion
DEX 유도 근위축 모델링 확립
EMS 및 SE의 독성평가
세포 보호 효과 평가
Fusion index 측정
Myotube diameter 측정
단백질 발현량 측정
Acknowledgement
국문요약
Conflict of interests
ORCID
References

영어 초록

This study aimed to investigate the protective effect of enzymatically modified stevia (EMS) on C2C12 cell-based model of dexamethasone (DEX)-induced muscle atrophy to provide baseline data for utilizing EMS in functional health products. C2C12 cells with DEX-induced muscle atrophy were treated with EMS (10, 50, and 100 μg/mL) for 24 h. C2C12 cells were treated with EMS and DEX to test their effects on cell viability and myotube formation (myotube diameter and fusion index), and analyze the expression of muscle strengthening or degrading protein markers. Schisandra chinensis Extract, a common functional ingredient, was used as a positive control. EMS did not show any cytotoxic effect at all treatment concentrations. Moreover, it exerted protective effects on C2C12 cell-based model of DEX-induced muscle atrophy at all concentrations. In addition, the positive effect of EMS on myotube formation was confirmed based on the measurement and comparison of the fusion index and myotube diameter when compared with myotubes treated with DEX alone. EMS treatment reduced the expression of muscle cell degradation-related proteins Fbx32 and MuRF1, and increased the expression of muscle strengthening and synthesis related proteins SIRT1 and p- Akt/Akt. Thus, EMS is a potential ingredient for developing functional health foods and should be further evaluated in preclinical models.

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