중금속 흡착능 유산균 분리
(주)코리아스칼라
- 최초 등록일
- 2023.06.19
- 최종 저작일
- 2019.06
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서지정보
ㆍ발행기관 : 한국유산균학회
ㆍ수록지정보 : Current Topic in Lactic Acid Bacteria and Probiotics / 5권 / 1호
ㆍ저자명 : 김자이, 김선규, 문기성
목차
Abstract
서 론
재료 및 방법
시약 및 재료
중금속(Ag, Cu, Zn) 내성 유산균주 선발
선발 유산균주의 중금속(Pb, Cd) 내성시험
선발 유산균주의 중금속(Pb, Cd) 흡착능 검증
Pb 제거능 정량분석
결 과
중금속(Ag, Cu, Zn) 내성 유산균주 선발
선발 유산균주의 중금속(Pb, Cd) 내성시험
선발된 유산균주의 Pb 및 Cd 흡착능 검증
선발균주의 Pb 제거능 정량분석
고 찰
References
영어 초록
Nowadays micro-dust is a serious problem in Korea. In particular micro-dust contains heavy metals such as Pb (lead) and Cd (cadmium) which negatively effect on human health. In this study, we intended to isolate lactic acid bacteria which can scavenge the heavy metals. Firstly we isolated two lactic acid bacteria which were resistant to Ag, Cu, and Zn (30-100 mM AgNO3, CuSO4, ZnSO4 in MRS broth). The two lactic acid bacteria CJNU 1877 and JG 15 were identified Lactobacillus paracasei and Enterococcus faecium, respectively. Subsequently the strains were inoculated in MRS broth and agar plate where 100 ppm of Pb(NO3)2 and CdSO4 were added, respectively. They did grow in the conditions and we found aggregations from 24 h cultures, indicating the strains can absorb the heavy metals, which was further proved by scanning electron microscopy (SEM) and Inductively Coupled Plasma-Optical Emission Spectrometer. Therefore the isolated lactic acid bacteria can be used as a probiotics harboring heavy metal scavenging activity.
참고 자료
없음
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