LC-MS/MS를 이용한 육류 중 아미노글리코사이드계 항생제 9종의 동시분석 및 적용성 검증

서지정보

ㆍ발행기관 : 한국식품위생안전성학회 ㆍ수록지정보 : 한국식품위생안전성학회지 / 29권 / 2호
ㆍ저자명 : 조윤제, 최선주, 김명애, 김미경, 윤수진, 장문익, 이상목, 김희정, 정지윤, 이규식, 이상재

목차

ABSTRACT
재료 및 방법
시료
시약 및 재료
표준원액 및 표준용액
추출 및 정제
LC-MS/MS 조건
분석법 검증
적용성 검증을 위한 잔류실태조사
결과 및 고찰
분석법 선정 및 조건 확립
분석법 검증
적용성 검증을 위한 잔류실태조사
요 약
참고문헌

영어 초록

A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~ 0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.

참고 자료

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