In Vitro Antibacterial Effect of the Combination of Galla rhois ethanol extracts and Sodium chlorate against Intramacrophage Brucella abortus
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- 2023.04.05
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- 2014.03
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서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 29권 / 1호
ㆍ저자명 : Gyeong-Hwan Kim, Il-Hwa Hong, Eun-Ah Yu, Eun-Kee Park, Chang-Yeol Yoo, Suk Kim, Hu Jang Lee
목차
ABSTRACT
Introduction
Materials and Methods
Cells and culture conditions
Bacterial culture
GRE and SC solution preparation
Cytotoxicity assay
Bactericidal analysis
Inhibitory effects for intracellular proliferation of B.abortus
Statistical analysis
Results
Cytotoxic effects on murine macrophage
Bactericidal effects of GRE and GS on B. abortus
Inhibitory effects of GRE, SC and GS on bacterialintracellular proliferation
Discussion
요 약
References
영어 초록
This study investigated the antibacterial effects of GR ethanol extracts (GRE), sodium chlorate (SC) and a combination of GRE and SC (GS) on Brucella abortus (B. abortus). The antibacterial activities of GRE, SC and GS towards B. abortus were evaluated by incubating B. abortus with GRE, SC and GS. Following treatment with GRE, SC and GS, B. abortus survival and intracellular proliferation in macrophages were monitored. In the cellular cytotoxicity assay, GRE, SC and GS are not cytotoxic at concentrations less than 400 μg/ml, 15 mM and 0.6GS (1 of GS, GRE 1,000 μg/ml + SC 30 mM), respectively. The viability of B. abortus was markedly decreased in a dosedependent manner in all treatment groups. In addition, B. abortus intracellular proliferation within macrophages was significantly reduced in cells treated with GRE (400 μg/mL), SC (15 mM) and 0.5GS (GRE 500 μg/mL + SC 15 mM) after 48 hr-incubation (GRE, p < 0.01; SC and 0.5GS, p < 0.001). Especially, in the treatment of GS, the synergistic effect of GRE and SC treatment on B. abortus in macrophage was observed. In conclusion, GS is useful as an antibacterial candidate against B. abortus, and can be applied in the field of meat and milk hygiene.
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