대장암 세포의 방사선저항성에 대한 p53의존성 PI3K의 역할

서지정보

ㆍ발행기관 : 한국식품위생안전성학회 ㆍ수록지정보 : 한국식품위생안전성학회지 / 25권 / 3호
ㆍ저자명 : 이희관, 김종석, 권형철

목차

ABSTRACT
재료 및 방법
재료
세포 수확 과 X-ray 방사선조사
Western blot 분석
세포 생존력 결정
데이타 분석
결 과
대장암세포에서 X-ray의 조사에 의한 p53 활성화
대장암세포에서 p53이 세포내 PI3-K 및 PTEN의 기저농도에미치는 영향
대장암세포에서 X-ray에 의하여 유도되는 PI3-K 및 PTEN의신호전달에 활성화에 미치는 p53의 영향
고 찰
참고문헌

영어 초록

Radiotherapy is one of the major therapies for cancer treatment. p53 acts as a central mediator of the cellular response to stressful stimuli, such as radiation. Recently it has been known that activation of the phosphatidylinositol- 3-kinase (PI3K) pathway is associated with radioresistance. In this study, we investigated whether X-irradiation up-regulates PI3K in a p53-dependent manner in human colon cancer cells. In order to study this phenomenon, we have treated p53-wild type and p53-mutant type HCT116 cells with X-ray. Treatment of wild type HCT116 cells with 8 Gy resulted in a marked increase in PI3K (p85), which paralleled an increase in PTEN, a counterpart of PI3K. However, these effects of X-rays in the p53-mutant cells were not observed. These results suggest that the X-irradiation- induced up-regulation of PI3K/PTEN pathway is p53-dependent.

참고 자료

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