식품중 Aflatoxin 측정방법의 비교
(주)코리아스칼라
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- 2023.04.05
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- 1996.06
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서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 11권 / 2호
ㆍ저자명 : 김명희
영어 초록
A procedure for the determination of Aflatoxins in food and grains which utilizes reversed phased liquid chromatographic (LC) analysis with postcolumn derivatization by an electrochemical cell and determination with a fluorescence detector has been evaluated. The LC mobile phase was water-acetonitrile-methanol (6+2+2) with 1mM KBr and 1 mM HNO₃ which gave baseline separation for the four Aflatoxins (AfB₁, AfB₂, AfG₁, AfG₂). The electrochemical cell set at 7V, generated bromine and derivatized aflatoxins B₁ and G₁, The derivatives were detected by the fluorescence detector. The aflatoxins in naturally contaminated corn samples were isolated by three different cleanup procedures: the AOAC method I column (CB method), a rapid filtrate column (Romer's column), and an immunoaffinity column. The final extract were quantitated with fluorodensitometric TLC and the LC postcolumn derivatization techniques. The results were quite similar, however the LC technique showed less interferences and could be automated. Samples of corn, raw peanuts, peanut butter and dried dates were also analyzed successfully with this procedure.
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