칡소와 흑우에서 MC4R gene SNP C1786T과 경제형질과의 연관성 분석
(주)코리아스칼라
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- 2023.04.05
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- 2013.06
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서지정보
ㆍ발행기관 : 강원대학교 동물자원공동연구소
ㆍ수록지정보 : 동물자원연구 / 24권 / 1호
ㆍ저자명 : 이영섭, 박새롬, 김훈, 김진우, 최소영, 이지연, 이준섭, 김기범, 박종운, 송영한, 이학교, 이성진
목차
ABSTRACT Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 공시재료 2. Genomic DNA 추출 3. MC4R 유전자 Primer 및 설계 4. MC4R 유전자의 PCR 증폭 5. 유전자형 분석 6. 통계분석 Ⅲ. 결론 및 고찰 1. MC4R gene SNP C1786T의 genotyping 2. 칡소와 흑우의 MC4R 유전자 SNP와 경제형질과의 관련성 분석 Ⅳ. 요약 Ⅴ. References
영어 초록
The melanocortin receptor type 4 (MC4R) gene is expressed in the hypothalamus and regulates energy intake and body weight. Recently, it has been reported that obesity and energy balance in human were also regulated by the MC4R gene. Therefore the objective of this study was to identify the polymorphism on the MC4R gene SNP C1786T and its association with economic traits in Korean native cattle (brindle and black cattle) by PCR-RFLP. A total of 125 cattle from the two breeds were tested for economic traits (meat quality index, backfat, thickness, carcass weight, longissimus muscle area and marbling score) and data was analyzed using SAS program. In the results, C allele had highest frequency than G allele frequency in the both breeds and the gene was significantly associated with meat quantity index and backfat thickness in brindle cattle breed. However, in black cattle, the gene was significantly associated with longissimus muscle area (p<0.05). These results suggest that C1786T SNP of the MC4R gene may be useful as a genetic marker for economic traits in the brindle and black cattle.
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