한우 체외수정란의 수정란이식에 관한 연구

서지정보

ㆍ발행기관 : 강원대학교 동물자원공동연구소 ㆍ수록지정보 : 동물자원연구 / 15권
ㆍ저자명 : 조명현, 박동헌, 장현용, 정희태, 박춘근, 김정익, 양부근

목차

ABSTRACT Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 한우 난포란의 체외성숙 및 수정 2. 체외수정란의 체외배양 3. 체외수정란의 동결보존 4. 한우 체외수정란의 이식 5. 임신감정 Ⅲ. 결과 1. 체외수정란의 체외발육 및 체외배양일수에 따른 수태율 2. 체외수정란의 발육상태에 따른 수태율과 이식 위치에 따른 수태율 3. 신선 및 동결란에 따른 이식 후 수태율 Ⅳ. 고찰 Ⅴ. 적요 Ⅵ. 인용문헌

영어 초록

These studies were carried out to improve the reproductive efficiency through embryos transfer of Hanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, Oocytes and zygotes were cultured for 40 to 44 h in CR1aa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and were cultured in CR1aa medium containing 10% fetal bovine serum and 2.5 mM taurine in 5% O2 and 5% CO2 at 38.5℃. The fresh and frozen thawed embryos of the morulae and blastocysts cultured for 6 to 9 days in vitro were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and 9 days in in vitro culture were 41.9, 48.6, 57.9 and 47.4%, respectively. In the developmental stage, pregnancy rates of early blastocysts (41.7%), blastocysts (57.6%) and expanded blastocysts (50.0%) were higher than that of morulae stage (00.0%). Fresh and frozen embryos on the pregnancy rates were 48.9 and 50.0%, respectively. These results indicate that the pregnancy rate after transfer were affected on embryonic stage of in vitro embryos and in vitro culture periods.

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