마이크로어레이를 이용한 봉독 처리된 돼지 초대배양 연골세포에서의 유전자 발현 프로파일링

서지정보

ㆍ발행기관 : 강원대학교 동물자원공동연구소 ㆍ수록지정보 : 동물자원연구 / 23권 / 2호
ㆍ저자명 : 박새롬, 이영섭, 김진우, 김 훈, 최소영, 이지연, 나레시 쿠마 싱, 오재돈, 이학교, 이성진

목차

ABSTRACT Ι. 서론 Ⅱ. 재료 및 방법 1. 관절 연골세포의 분리 및 배양 2. 봉독이 연골세포에 미치는 영향, 배양 및 봉독 처리 3. RNA추출 및 Microarry분석 Ⅲ. 결론 및 고찰 Ⅳ. 요약 Ⅴ. 인용문헌

영어 초록

Osteoarthritis is one of the commonest causes associated with age-related damage of articular cartilage. Non-steroidal anti-inflammatory drugs are commonly used in osteoarthritic patient. However, long term administration of these drugs results gastrointestinal disorders. Though, most studies have demonstrated in the past that bee venom has therapeutic effect on diseases related to inflammation and pains, but its anti-inflammatory properties have not been so far studied on inflamed chondrocytes (LPS induced) invitro. For the purpose, the study was carried out to determine the effect of bee venom on porcine articular chondrocyte cell using microarray. In this study, we found that 2,235 significantly associated gene (1,404 up-regulated genes and 831 down-regulated genes) that were expressed on inflamed and non inflamed chondrocytes during proliferation. Among the 1,404 up-regulated genes and 831 down-regulated genes, known genes were 372 and 237, respectively. On the other hand, bee venom significantly reduced expression of fetuin involved in acute inflammatory reaction. Our results suggest that this study could be useful database in gene expression profiling of chondrocyte cell treated with bee venom.

참고 자료

없음