Antibody-based Screening of Porphyromonas gingivalis Proteins Specifically Produced in Patients with Chronic Periodontitis
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- 2023.04.03
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- 2018.12
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 43권 / 4호
ㆍ저자명 : Hye-Jung Kim, Seok-Woo Lee
목차
Introduction
Materials and Methods
Bacterial strains and growth conditions
Construction of genomic expression libraries of P.gingivalis ATCC 33277
Collecting dental plaque samples containing P.gingivalis and preparing hyperimmune antisera
Serum adsorption
Screening for recombinant proteins uniquely expressed in vivo
Identifying genes isolated by CMAT
Predicting functions of antigens identified by CMAT
Results
Adsorption of rabbit hyper-immune antisera
Genomic library construction and screening
Identification and functional classification of P.gingivalis genes identified by CMAT
Discussion
References
영어 초록
Porphyromonas gingivalis is among the major etiological pathogens of chronic periodontitis. The virulence mechanisms of P. gingivalis is yet to be identified as its activity is largely unknown in actual disease process. The purpose of this study is to identify antigens of P. gingivalis expressed only in patients with chronic periodontitis using a unique immunoscreening technique. Change Mediated Antigen Technology (CMAT), an antibody-based screening technique, was used to identify virulence-associated proteins of P. gingivalis that are expressed only during infection stage in patients having chronic periodontitis. Out of 13,000 recombinant clones screened, 22 tested positive for reproducible reactivity with rabbit hyperimmune anti-sera prepared against dental plaque samples acquired from periodontitis patients. The DNA sequences of these 18 genes were determined. CMAT-identified protein antigens of P. gingivalis included proteins involved in energy metabolism and biosynthesis, heme and iron binding, drug resistance, specific enzyme activities, and unknown functions. Further analysis of these genes could result in a novel insight into the virulence mechanisms of P. gingivalis.
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