Immunogenicity of a bacterial ghost delivered dengue virus serotype 2 envelope protein domain III followed by an intramuscular protein boosting strategy in mice model

서지정보

ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회) ㆍ수록지정보 : 예방수의학회지 / 46권 / 3호
ㆍ저자명 : Gayeon Won, Irshad Ahmed Hajam, Perumalraja Kirthika, Eunha Kim, John Hwa Lee

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Ethics statement
Animals, virus and cell lines
Production of Salmonella typhimurium BGs expressingDENV2 EDIII domain
Immunization and challenge studies in C57BL/6 mice
Measurement of antibody titers, cross reactivity andavidity
Antigen-specific T cell responses
Statistical analyses
RESULTS
Confirmation of EDIII protein expressed by BGs
BG-EDIII vaccine induces efficient DENV2-specifichumoral and cell-mediated immune responses
BG-EDIII vaccine inhibits DENV2 viremia l
DISCUSSION
CONCLUSION
ACKNOWLEDGEMENTS
DECLARATION OF INTEREST
REFERENCES

영어 초록

This study aimed to investigate whether bacterial ghosts (BGs), empty cell envelopes of a gram-negative bacterium, delivering envelope protein domain III (EDIII) of dengue virus (DENV) serotype 2 could induce protective immune responses against dengue infection. In this study, we constructed Salmonella Typhimurium BGs expressing and delivering EDIII (BG-EDIII) and evaluated these ghosts for their immunogenicity studies in C57BL/6 mice. Our results demonstrated that the mice vaccinated once orally with BG-EDIII followed by an intramuscular boosting with a recombinant EDIII protein elicited significantly higher humoral and cell-mediated immune responses compared to the BGs alone vaccinated group (p<0.001). Upon challenge with DENV2, significantly lower viral load and liver damage was observed in BG-EDIII vaccinated group than BGs alone control group (p<0.05). The outcomes of this study revealed the ability of BG- EDIII to stimulate immune response with no observable damage to the vital organs.

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