Disruption of the relA and spoT genes in enteropathogenic Escherichia coli E2348/69 reduces bacterial persistence in a porcine gastrointestinal tract

서지정보

ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회) ㆍ수록지정보 : 예방수의학회지 / 46권 / 4호
ㆍ저자명 : Jun Bong Lee, Jang Won Yoon

목차

INTRODUCTION
MATERIALS AND METHODS
Bacterial strains
Animal challenge and fecal sampling
Detection of E2348/69 strains in feces by PCR
Tissue preparation and histologic assessment
RESULTS
Mutation of the relA and spoT genes in EPEC leads torapid bacterial clearance in porcine GIT
The double mutant of E2348/69 cannot inducepathologic lesions in pig
DISCUSSION
ACKNOWLEDGMENTS
REFERENCES

영어 초록

Enteropathogenic Escherichia coli (EPEC) is one of the etiological agents that causes diarrhea in weaning pigs. In this study, we report that mutating both relA and spoT genes in EPEC E2348/69 can promote bacterial clearance in porcine gastrointestinal tract (GIT). Our experimental analyses showed that an E2348/69 ΔrelAΔspoT mutant strain was not detected in porcine feces after 1 day post-infection (dpi), whereas its parental strain was continuously detected in porcine feces within 10 dpi. Histologic assessment revealed that the mutant strain was unable to induce moderate pathologic lesions in porcine GIT when compared to those with the wildtype strain. Taken together, our data suggest that the relA and spoT genes in EPEC play an important role in bacterial survival and pathogenesis in porcine GIT.

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