동충하초(Cordyceps militaris) 균사체 액체발효 포스트바이오틱스로부터 항염증 활성다당 분리
(주)코리아스칼라
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- 2023.04.03
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- 2023.02
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서지정보
ㆍ발행기관 : 한국식품영양학회
ㆍ수록지정보 : 한국식품영양학회지 / 36권 / 1호
ㆍ저자명 : 김연숙, 신현영, 김훈, 정은진, 김현경, 서민근, 서형주, 유광원
목차
Abstract
서 론
재료 및 방법
1. 동충하초 균사체 액체발효 및 포스트바이오틱스 제조및 분획
2. 세포주 및 배양조건
3. RAW 264.7 대식세포를 이용한 항염증 활성
4. HaCaT 세포를 통한 항염증 활성
5. 분자량 분포
6. 구성분 및 구성당 분석
7. β-1,3/1,6-glucan 함량 분석
8. 통계처리
결과 및 고찰
1. 동충하초 균사체 액체발효물 유래 포스트바이오틱스분획획분의 세포독성
2. RAW 264.7 세포주를 이용한 염증 억제
3. HaCaT 세포를 이용한 피부염 억제 활성
4. 분자량 분석
5. 구성분 및 구성당 분석
6. β-1,3/1,6-glucan 분석
요약 및 결론
감사의 글
References
영어 초록
To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.
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