Characterization of Mouse Interferon-Induced Transmembrane Protein-1 Expression in Mouse Testi

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 3호
ㆍ저자명 : Ran Lee, Hyun Jung Park, Won Young Lee, Ji Hyuk Kim, In Chul Kim, Dong Woon Kim, Sung Dae Lee, Hyun Jung Jung, Jong Moon Kim, Hyung Moon Yoon, Hyuk Jung Kwon, Hyuk Song

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Immunohistochemistry Immunoblotting Analysis of Sperm Parameters Induction of Capacitation and PSA Staining Sperm-Zona Pellucida Free Oocyte Binding Assay Statiscal Analysis RESULT AND DISCUSSION Ifitm-1 Expressed in Spermatozoa in Adult Testes Ifitm-1 Expression Was not Altered by Acrosome Reaction Effects of Ifitm-1 on Sperm Motility and Fertility REFERENCES

영어 초록

Interferon induced transmembrane protein-1 (Ifitm-1) has been reported to have an important role in primordial germ cell formation, and it has expressed in female reproductive organ. In the present study, Ifitm-1 gene expression was identified in testes and all part of epididymis using western immunoblot and immunohistochemistry. Interestingly, Ifitm-1 expression was observed on the head of spermatozoa. To investigate the role of Ifitm-1 gene expression in behavior of spermatozoa after acrosome reaction, fresh sperm was incubated with calcium ionophore to induce acrosome reaction, whereas the expression of Ifitm-1 was not altered after the acrosome reaction. Then to identify the effect of Ifitm-1 in sperm motility and other seminal parameters, different concentration of Ifitm-1 antibody was incubated with spermatozoa, and seminal parameters were assessed using computer-assisted semen analysis (CASA). Interestingly, motility, progressive, and VAP were increased in the sperm with Ifitm-1 antibody treated compared to rabbit serum, however other parameters such as straightness were not changed. In order to identify the functional significance of Ifitm-1 in fertilization, capacitated spermatozoa were pre-incubated with anti- Ifitm-1 antibody and subsequently examined the ability to adhere to mouse oocytes. However, any defection or alteration in sperm-egg fusion was not found, Ifitm-1 antibody treated or non-treated spermatozoa showed a normal penetration. Although the precise role of Ifitm-1 in sperm motility and following fertilization need to be elucidated, this study suggests that the activation of Ifitm-1 on the sperm may enhance the motility of spermatozoa in mice.

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