Involvement of Ski Protein Expression in Luteinization in Rat Granulosa Cells
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : Hyun Kim, Takashi Matsuwaki, Keitaro Yamanouchi, Masugi Nishihara, Sungwoo Kim, Yeoung-Gyu Ko, Boh-Suk Yang
ㆍ저자명 : Hyun Kim, Takashi Matsuwaki, Keitaro Yamanouchi, Masugi Nishihara, Sungwoo Kim, Yeoung-Gyu Ko, Boh-Suk Yang
목차
INTRODUCTION MATERIALS AND METHODS Animals Hormone Assay Immunohistochemistry and TUNEL Staining qPCR (Real Time PCR) Statistical Analysis RESULTS DISCUSSION REFERENCES영어 초록
Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinization to predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally.참고 자료
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