[자연과학]gene cloning ch2 플라스미드와 박테리오파지

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목차

2장,Vehicles for Gene Cloning : Plasmids and Bacteriophages

2.1 Plasmid
2.1.1 Basic features of Plasmids
2.1.2 size and copy number
2.1.3 Conjugation and compatibility (접합과 조합성)
2.1.4 Plasmid classification
2.1.5 Plasmids in organisms other than bacteria

2.2 Bacteriophages
2.2.1 Basic features of bacteriophages
2.2.2 Lysogenic phages(λ phage, M13 phage)
2.2.3 다른 생물체를 위한 클로닝 벡터로서의 바이러스

본문내용

- lytic cycle.
: With some phage types the entire infection cycle is
completed very quickly, possibly in less than 20minutes.

1. The phage attaches to the bacterium and injects its DNA
2. The phage DNA molecule is replicated
3. capsid components are synthesized, new phage particles
are assembled and released.

2.2.2 Lysogenic phages(λ phage, M13 phage)

- Lysogenic infection is characterized by retention of the phage
DNA molecule in the host bacterium, possibly for many
thousands of cell divisions.

- lysogenic phage DNA is inserted into the bacterial genome.
The integated form of the phage DNA(called the prophage) is
quiescent, and a bacterium (reffered to as a lysogen) that
carris a prophage is usually physiologicaaly indistinguishable
from an uninfected cell.

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