[HPLC] RP-HPLC & Case Study

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HPLC에 관한 Powerpoint 문서 입니다..
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목차

HPLC
Base Material for HPLC
Normal-Phase Chromatography
Reverse-Phase Chromatography
a.HPLC Solvent Selection
Chromatogram-Selectivity and Resolution
High Performance Chromatography
Peptide Production (Univ. Cambridge)
Peptide Productio
a.Aims
b.Background
Peptide Production (Univ. Cambridge)
a.Design of Fusion Tag
b.Key Factors
c.Cleavage
d.KSI-Lac21-His6
e.CDAP Cyanylation
f.Ammonium Cleavage
g.Confirmation Using RP-HPLC
h.Cation Exchange Chromatography
i.RP-HPLC Purification
j.Comparison of Synthetic & Modified Lac21s
k.Characterization of mLac21 ?Interfacial Tension
l.Characterization of mLac21 ?Circular chroism

What is Protein?
Structure of Heparin
Structure of Sephadex

본문내용

Reverse-Phase Chromatography
 Stationary phase : silica- n-butyl, n-octyl, n-octyldecyl
 Mobile phase : polar solvent (acetonitrile, methanol)
 Application
- soluble in polar solvent (water)

Column selection : hydrophobicity, protein size
- hydrophobic, > 50 kDa : C4
- middle, 10 ~ 50 kDa : C8
- hydrophilic, < 20 kDa : C18

How can we enhance selectivity?
- ion-paring agent : 0.1% TFA (tetrafluoroacetic acid)
- buffer : phosphate buffer

참고 자료

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