유산균을 이용한 유청 바이오컨버전 산물의 병원성 세균 항생물막 효과

서지정보

ㆍ발행기관 : 한국유산균학회 ㆍ수록지정보 : Current Topic in Lactic Acid Bacteria and Probiotics / 6권 / 1호
ㆍ저자명 : 이진호, 김민주, 김동한, 유창엽, 김봉선, 이지수, 강석성

목차

Abstract
서 론
재료 및 방법
균주 및 시약
유청 바이오컨버전 제조
병원균 생물막의 형성 억제 분석
병원균 생장 억제 분석
통계분석
결 과
유청 바이오컨버전 산물에 의한 E. coli O157:H7의 생물막형성 억제
유청 바이오컨버전 산물에 의한 L. monocytogenes의 생물막형성 억제
유청 바이오컨버전 산물에 의한 병원균의 생장 억제
고 찰
References

영어 초록

Whey is a major by-product of cheese manufacture and contains many valuable constituents, such as β-lactoglobulin, lactoferrin and immunoglobulin. The current study determined the anti-biofilm activity of bioconversion of whey by Lactobacillus plantarum (LP-W), L. rhamnosus GG (LR-W), L. brevis (LB-W) and Enterococcus faecium (EF-W) against foodborne pathogenic bacteria, Escherichia coli O157:H7 and Listeria monocytogenes. When the foodborne pathogenic bacteria were co-incubated with LP-W, LR-W, LB-W or EF-W, biofilms by E. coli O157:H7 and L. monocytogenes were significantly reduced by all bioconversion of whey. Moreover, LP-W, LR-W, LB-W and EF-W also dramatically reduced pre-formed biofilm by E. coli O157:H7 and L. monocytogenes, suggesting that the bioconversion of whey effectively suppress the development and disruption of biofilm by foodborne pathogenic bacteria. Furthermore, in order to determine the growth kinetics of E. coli O157 and L. monocytogenes planktonic cells in the presence the bioconversion of whey, LP-W, LR-W, LB-W and EF-W did not significantly inhibited the growth of foodborne pathogenic bacteria, implying that the bioconversion of whey reduces the biofilm without the decrease of bacterial growth. Taken together, these results suggest that bioconversion of whey by lactic acid bacteria could be a promising agent for the reduction of microbial biofilm.

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