Streptococcus thermophilus KCTC14471BP의 안전성 평가 및 실크 피브로인 코팅의 면역 증진 효능

서지정보

ㆍ발행기관 : 한국유산균학회 ㆍ수록지정보 : Current Topic in Lactic Acid Bacteria and Probiotics / 8권 / 1호
ㆍ저자명 : 권기철, 허보혜, 손성호, 권유진, 권미진, 장주현, 신혜정, 김인수, 박성엽, 김경환, 김병국, 신창훈, 김병용

목차

Abstract
서 론
재료 및 방법
유산균 분리 및 배양
유산균의 실크 피브로인 코팅
효소 활성 평가
항생제 감수성 평가
Mucin 코팅판 부착능 시험
세포 배양
MTT assay 시험
Real-time PCR 측정
Enzyme-linked immunosorbent assay(ELISA) 확인
통계 분석
결 과
S. thermophilus KCTC14471BP의 효소 활성
S. thermophilus KCTC14471BP의 항생제 감수성 확인
S. thermophilus KCTC14471BP의 mucin binding ability에서실크 피브로인 코팅 효과
실크 피브로인 코팅 S. thermophilus KCTC14471BP의 세포독성 평가
실크 피브로인 코팅 S. thermophilus KCTC14471BP의 면역활성 효과
실크 피브로인 코팅 S. thermophilus KCTC14471BP의 농도의존적 면역 활성 확인
고 찰
요 약
References

영어 초록

Probiotic strain is known to regulate the immune system by colonizing in the intestine and interact with intestinal cell receptors of lymphoid tissue. In this study, safety of Streptococcus thermophilus KCTC14471BP and silk fibroin coating effects was evaluated with respect to mucin binding abilities and immune system modulation. S. thermophilus KCTC14471BP was coated with silk fibroin by adding 1% water-soluble calcium and 0.1% silk fibroin. S. thermophilus KCTC14471BP showed the high activities of leucine arylamidase and β-galactosidase. Regarding the antibiotic resistance tests, S. thermophilus KCTC14471BP was susceptible to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol. S. thermophilus KCTC14471BP coated with silk fibroin showed the improved mucin binding ability from 16.1% to 71.3% and was confirmed to have no cytotoxicity against RAW 264.7 macrophage. S. thermophilus KCTC14471BP coated with silk fibroin showed dose-dependently significant increases in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. These results suggested that S. thermophilus KCTC14471BP can be expected as a promising probiotic bacteria for applications to food industries such as fermentation or functional foods.

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