Brazilin Inhibits of TPA-induced MMP-9 Expression Via the Suppression of NF-κB Activation in MCF-7 Human Breast Carcinoma Cells
(주)코리아스칼라
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- 2023.04.05
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- 2010.09
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서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 25권 / 3호
ㆍ저자명 : Byeong-Soo Kim
목차
ABSTRACT
Introduction
Materials and Methods
Cells and Material
Determination of cell viability
Western blot analysis
Gelatin Zymography assay
Quantitative real-time PCR assay
Preparation of nuclear extract
Electrophoretic mobility shift assay (EMSA)
Invasion assay
Results
Effect of brazilin on of MCF-7 cell viability
Brazilin inhibits TPA-induced MMP-9 expression inMCF-7 cells
Brazilin suppresses TPA-induced NF-κB DNA bindingactivity in MCF-7 cells
Brazilin inhibits TPA-induced invasion of MCF-7cells
Discussion
References
영어 초록
Metastasis is the primary cause of from breast cancer mortality. Cell migration and invasion play important roles in neoplastic metastasis. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. NF-κB is transcription factor important in the regulation of MMP-9, as the promoter of MMP-9 gene contains binding sites for NF-κB. Brazilin, an active component of sappan wood (Caesalpinia sappan), decreases TPA-induced MMP-9 expression and invasion in MCF-7 cells. Also, brazilin suppressed NF-κB activation in TPA-treated MCF-7 cells. Taken together, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by brazilin is mediated by the suppression of the NF-κB pathway in MCF-7 cells. This result suggest brazilin provide a potential therapeutic approach for the treatment of breast cancer.
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