Latex of Ficus carica L. Induces Apoptosis Through Caspase and Bcl-2 Family in FaDu Human Hypopharynx Squamous Carcinoma Cells

서지정보

ㆍ발행기관 : 대한구강생물학회 ㆍ수록지정보 : International Journal of Oral Biology / 42권 / 4호
ㆍ저자명 : Bo Su Shin, Seul Ah Lee, Sung Min Moon, Seul Hee Han, Eun Ju Hwang, Su-Gwan Kim, Do Kyung Kim, Jin-Soo Kim, Bo-Ram Park, Chun Sung Kim

목차

Introduction
Materials and Methods
Preparation of latex from Ficus carica L.
Reagents
Cell culture
SDS-PAGE
Gelatinolytic activity
Cytotoxicity
Flow cytometry analysis
Western blot analysis
Statistical Analysis
Results
Effect of Latex on viability of FaDu cells.
Induction of apoptosis by latex in FaDu cells.
Activation of caspase and Bcl-2 family by latex inFaDu cells.
Discussion
Conflict of interest
Acknowledgments
References

영어 초록

Ficus carica L. (common fig), one of the first plants cultivated by humans, originated in the Mediterranean basin and currently grows worldwide, including southwest Asia and South Korea. It has been used as a traditional medicine for treatment of metabolic, cardiovascular, and respiratory diseases as well as hemorrhoids and skin infections. Its pharmacological properties have recently been studied in detail, but research on the anti-cancer effect of its latex has been only been studied on a limited basis on several cell lines, such prostate cancer, breast cancer, and leukemia. In this study, we investigated the anti-cancer activity of the latex of Ficus carica L.and its underlying mechanism in FaDu human hypopharynx squamous carcinoma cells. (See Ed. note above) We confirmed through SDS-PAGE analysis and gelatinolytic activity analysis that the latex of Ficus carica contains cysteine protease ficin. Our data showed that the latex inhibited cell growth in a dose-dependent manner. In addition, the latex treatment markedly induced apoptosis in FaDu cells as determined by FACS analysis, elevated expression level of cleaved caspase-9, -3 and PARP (poly (ADP-ribose) polymerase), and. increased the expression of Bax (pro-apoptotic factor) while decreasing the expression of Bcl-2 (anti-apoptotic factor). Taken together, these results suggested that latex containing the ficin inhibited cell growth and induced apoptosis by caspase and the Bcl-2 family signaling pathway in FaDu human hypopharynx squamous carcinoma cells. These findings point to the potential of latex of Ficus carica to provide a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.

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