Anticancer effects of D-pinitol in human oral squamous carcinoma cells
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 45권 / 4호
ㆍ저자명 : Hyun-Chul Shin, Tea-Hyun Bang, Hae-Mi Kang, Bong-Soo Park, In-Ryoung Kim
ㆍ저자명 : Hyun-Chul Shin, Tea-Hyun Bang, Hae-Mi Kang, Bong-Soo Park, In-Ryoung Kim
목차
IntroductionMaterials and Methods
1. Drug and reagents
2. Cell culture
3. Cell viability assay
4. Colony formation assay
5. Hoechst 33342 staining assay
6. Wound healingassay
7. Transwell invasion assay
8. Flow cytometry
9. Western blot analysis
10. Statistical analysis
Results
1. D-pinitol reduced the cell viability and proliferationin OSCC cells
2. D-pinitol induced apoptosis in OSCC cells
3. D-pinitol inhibited the cell migration and invasion inOSCC cells
4. D-pinitol regulated the EMT-related proteinexpression in OSCC cells
5. D-pinitol induced G1 cell arrest in OSCC cells
Discussion
Acknowledgements
Conflicts of Interest
References
영어 초록
D-pinitol is an analog of 3-methoxy-D-chiro-inositol found in beans and plants. D-pinitol has anti-inflammatory, antidiabetic, and anticancer effects. Additionally, D-pinitol induces apoptosis and inhibits metastasis in breast and prostate cancers. However, to date, no study has investigated the anticancer effects of D-pinitol in oral cancer. Therefore, in this study, whether the anticancer effects of D-pinitol induce apoptosis, inhibit the epithelialto- mesenchymal transition (EMT), and arrest cell cycle was investigated in squamous epithelial cells. D-pinitol decreased the survival and cell proliferation rates of CAL-27 and Ca9-22 oral squamous carcinoma cells in a concentration- and time-dependent manner. Evidence of apoptosis, including nuclear condensation, poly (ADP-ribose) polymerase, and caspase-3 fragmentation, was also observed. D-pinitol inhibited the migration and invasion of both cell lines. In terms of EMT-related proteins, E-cadherin was increased, whereas N-cadherin, Snail, and Slug were decreased. D-pinitol also decreased the expression of cyclin D1, a protein involved in the cell cycle, but increased the expression of p21, a cyclin-dependent kinase inhibitor. Hence, D-pinitol induces apoptosis and cell cycle arrest in CAL-27 and Ca9-22 cells, demonstrating an anticancer effect by decreasing the EMT.참고 자료
없음태그
"International Journal of Oral Biology"의 다른 논문
- Role of microglial activation on neuronal excitability ..7페이지
- Antitumor effects of octyl gallate on hypopharyngeal ca..7페이지
- Induction of anti-aquaporin 5 autoantibodies by molecul..7페이지
- High-salt and glucose diet attenuates alveolar bone rec..7페이지
- Increased SOX2 expression in three-dimensional sphere c..7페이지
- Alteration of cellular events in tooth development by c..7페이지
- Green tea polyphenol (–)-epigallocatechin-3-gallate pre..11페이지
- L-ascorbic acid induces apoptosis in human laryngeal ep..10페이지
- High concentration of calcium represses osteoblast diff..7페이지
- Inhibitory effects of Coptis chinensis extract on the g..9페이지