Establishment of withdrawal time and analysis of chlorpheniramine maleate residue in milk of intramuscularly administered dairy cows

서지정보

ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회) ㆍ수록지정보 : 예방수의학회지 / 46권 / 2호
ㆍ저자명 : Chun-Nam Cha, Jonghyup Kim, Suk Kim, Ki-Rim Kim, Seunghee Ki, Hu-Jang Lee

목차

Abstract
서 론
재료 및 방법
시약 및 재료
실험동물
시험약제 투여 및 시료채취
표준원액 및 표준용액의 조제
시료 전처리
기기분석조건
분석법 검증
우유 중 CPM의 잔류휴약기간 설정
결 과
CPM의 크로마토그램
검량곡선, 검출한계 및 정량한계
회수율과 정밀성
우유 중 CPM 잔류 분석
우유 중 CPM 잔류휴약기간 설정
고 찰
감사의 글
REFERENCES

영어 초록

This study investigated chlorpheniramine maleate (CPM) residues in milk of intramuscularly dosed dairy cows and established the withdrawal time (WT) of CPM in milk. Sixteen healthy Holstein cows were injected with 10 (CPM-1) and 20 mL (CPM-2) of the drug containing 4 mg/mL of CPM, respectively. After administration of CPM, milk samples were collected from all cows at 12 hour intervals for 5 days. CPM residue concentrations in milk were determined using LC-MS/MS. The correlation coefficient of the calibration curve was 0.9956, and the limits of detection and quantification (LOQ) were 0.6 and 1.0 μg/kg, respectively. Recoveries ranged between 98.5-115.0%, and coefficient of variations were less than 10.96%. After treatment, CPM in CPM-1 and CPM-2 was detected below the LOQ in all milk samples at 12 hours. According to the European Medicines Agency’s guideline on determination of withdrawal periods for milk, withdrawal periods of both CPM-1 and CPM-2 in milk were established to 12 hours. In conclusion, the developed analytical method is sensitive and reliable for detection of CPM in milk, and the estimated WT of CPM in bovine milk will contribute to ensuring the safety of milk.

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