Differential Expression Screening of Defense Related Genes in Vitis flexuosa Grapevine against Elsinoe ampelina and Rhizobium vitis

서지정보

ㆍ발행기관 : 한국국제농업개발학회 ㆍ수록지정보 : 한국국제농업개발학회지 / 24권 / 4호
ㆍ저자명 : Soon-Young Ahn, Seon Ae Kim, Seung Heui Kim, Hae KeunYun

한국어 초록

국내야생종인 새머루로부터 내병성 포도품종 육성에 필요한 육종소재와 유용정보를 획득하고자, 포도 새눈무늬병균과 줄기혹병균에 감염된 새머루에서 식물 방어관련유전자의 발현양상을 분석하였다. Thaumatin (TLP), glutathione peroxidase (GPX), glutathione-S-transferase (GST), chalcone synthesis (CHS), protein kinase regulator (14-3-3) 및 β-glucanase (Glu) 등의 방어관련유전자, leucine reach-repeat (LRR)와 lipoxygenase (LOX) 등의 신호전달관련유전자, polygalacturonase-inhibiting protein (PGIP)과 같은 세포벽관련 유전자의 발현이 포도 새눈무늬병균 접종에 의해 유도되었다. 포도새눈무늬병균의 포자접종 및 배양여액처리에 의해 WRKYtranscription factor 10 (WRKY), fatty acid elongase (FAE)의 발현이 유도되었으며 proline-rich protein (PRP) 유전자의발현은 억제되었다. 발현이 증가한 15개 유전자 중에서 LRR, LOX, TLP, GST 등은 포도새눈무늬병균 포자현탁액 접종에 의해 발현이 크게 증가하였으나, CHS와 tonoplast intrinsicprotein (TIP)는 배양여액처리에 의해 증가하였다. 포도 줄기혹병균의 접종에 의해 LRR, CLP, LOX, TLP, GPX, 14-3-3,GST, PGIP, FAE, TIP, Glu, WRKY 등은 발현이 증가하였고, CHS와 PRPs는 발현이 억제되었다. 포도 줄기혹병균 접종에 의해 활성산소의 축적 및 분해와 관련된 GPX와 GST는 발현이 크게 증가하였다.

영어 초록

To collect useful genetic resources and information for disease resistant grape breeding from Korean wild grape, various genes related with defense responses were screened for their differential expressions in Vitis flexuosa grapevines against Elsinoe ampelina and Rhizobium vitis. The expressions of plant defense-related genes such as thaumatin-like protein (TLP), glutathione peroxidase (GPX), glutathione-S-transferase (GST), chalcone synthesis (CHS), protein kinase regulator (14-3-3), and â-glucanase-like protein (Glu), and signal transduction-related genes such as leucine reach-repeat (LRR) and lipoxygenase (LOX), and cell wall modification-related gene such as polygalacturonase-inhibiting protein (PGIP) were induced by E. ampelina inoculation. WRKY transcription factor 10 (WRKY), fatty acid elongase (FAE), tonoplast intrinsic protein (TIP), and meiosis 5 (Mei5) were up-regulated and proline-rich protein (PRPs) were down-regulated by both inoculation of spores and treatment of culture filtrates (CF) of E. ampelina. Among 15 up-regulated genes, LRR, LOX, TLP, and GST were specifically up-regulated by spore inoculation than CF treatment. On the contrary, CHS and TIP were highly upregulated by CF treatment than spore inoculation of E. ampelina. While LRR, CLP, LOX, TLP, GPX, 14-3-3, GST, PGIP, FAE, TIP, Glu, Mei5, and WRKY were up-regulated, CHS and PRPs were downregulated by R. vitis inoculation. The defense genes related with active oxygen species such as GPX and GST were highly activated in grapevine leaves with inoculated R. vitis.

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