Effect of MLN8237, a Novel Aurora A Kinase Inhibitor, on the Spontaneous Fragmentation of Ovulated Mouse Oocytes
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Jihun Park, Taesaeng Choi
ㆍ저자명 : Jihun Park, Taesaeng Choi
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Oocyte Collection and Culture Evaluation of the Oocytes Measurement of cdk1 Immunoblot Analysis of cyclin B1 Statistical Analysis RESULTS Effect of MLN8237 on Oocyte Fragmentation Levels of cyclin B and cdk1 Activity in MLN-treated Oocytes DISCUSSION REFERENCES영어 초록
Aurora A kinase is a mitotic serine/threonine kinase whose proposed functions include the maturation of centrosomes, G2/M transition, alignment of chromosomes at metaphase, and cytokinesis. In this study, we investigated the effect of MLN8237, an aurora A kinase inhibitor, on the postovulatory aging of oocytes based on the frequency of oocyte fragmentation, cdk1 kinase activity, and cyclin B degradation. The fragmentation of ovulated oocytes during prolonged culture was inhibited by treatment with MLN8237 in a concentration-dependent manner. The frequency of fragmented oocytes was significantly lower in oocytes treated with 2 μM MLN8237 (13%) than in control oocytes (64%) after two days of culture. Most of the control (non-fragmented) oocytes (91%) were activated after two days of culture. In comparison, only 22% of the MLN8237-treated oocytes were activated; the rest of the oocytes (78%) were still in metaphase with an abnormal spindle and dispersed chromosomes. Next, cdk1 activity and the level of cyclin B were examined. The level of cyclin B and cdk1 activity in MLN8237-treated oocytes were nearly equal to those in control oocytes. Our results indicate that MLN8237 inhibited the fragmentation of ovulated oocytes during prolonged culture, although it blocked the spontaneous decrease in activity of cdk1 and degradation of cyclin B. This mechanism of inhibition is different from that in oocytes treated with nocodazole, which have high levels of cdk1 activity and cyclin B.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
- GH Increases the Progesterone at Peri-estrus Stage in M..7페이지
- Alpha 1,3-Galactosyltransferase Deficiency in Miniature..8페이지
- Toxicity and Biomedical Imaging of Fluorescence-Conjuga..8페이지
- Identification and Characterization of Bull Sperm Hyalu..8페이지
- Generation of Reactive Oxygen Species and Subsequent DN..5페이지
- Effects of Escherichia coli Contamination on Extended P..5페이지
- Effect of Activation of Porcine NT Embryos on In Vitro ..4페이지
- Effects of Sodium Nitroprusside and Nitric Oxide on In ..5페이지
- Changes in Plasminogen Activity in Uterus Tissue during6페이지
- The Effect of Jazf1 Overexpression in Zebrafish Cardiac..5페이지