Principle 1) ROS-Glo H2O2 AssayROS-Glo H2O2 Assay는 ROS 중에서도 가장 반감기가 긴 H2O2를 측정할 수 있는 방법이다. ... Title : ROS detection Assay 2. Purpose : ROS를 세포독성지표로 사용하여, CCCP 처리 조건에 따른 5-FU의 세포독성을 파악한다. 3. ... Discussion 본 실험에서는 CytoTox-Glo Cytotoxicity Assay를 이용하여 MC38 cell에서 CCCP 처리군과 비처리군에서 5-FU 처리 시의 ROS level을
항산화 평가를 위한 ROS Assay 실험 제목 : 항산화 평가를 위한 ROS Assay 실험 과정 실험에 사용한 세포 : cell line Beas B2 (사람유래 기관지 세포) ... ROS assay (DCFH-DA treat) LPS 처리 12시간 후 ROS를 측정하기 위해 DCFDA 2µM을 HBSS medium에 희석시켜 줍니다. ... 억제 효과에 대한 항산화 억제 assay를 진행하였다.
황산화 효과를 DCFH-DA assay로 관찰한다. (2) 실험의 배경(DCFH-DA assay) DCFH-DA는 세포 내 활성산소종(ROS)의 표지자 역할을 한다. ... 예상되는 실험의 결과로는 활성산소종을 detect하는 DCFH-DA assay의 성질에 따라 t-BHP만 처리했을 경우 많은 형광이, ROS를 억제하는 sulforaphane을 함께 ... DCFH-DA assay로 관찰하였다.
antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay ... data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS ... During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS
및 지방축적과 ROS 생성 억제효과를 관찰하였다. ... 활성의 차이를 구명하고자 수행 하였다. 2016년 6월, 7월 그리고 8월에 수확한 고려 엉겅퀴를 이용하여 지표성분 분석, 총 플라보노이드 함량, DPPH 라디칼 소거활성, ORAC assay ... content, total flavonoids content and antioxidant effects (DPPH radical scavenging activity and ORAC assay
assay를 진행한 결과, ROCK kinase가 이에 관여한다는 것을 알아냈다. ... 다음으로는 cellular senescence에서 증가하는 ROS를 보고 ROS를 감소시키면 역노화가 가능할 것이라고 생각했고, ROS를 detection할 수 있는 방법으로 kinase ... 이 ROCK을 억제하면 ROS는 감소하였고, mitochondrial membrane potential이 개선되는 것을 확인하였다.
Intacellular ROS detection / Griess assay 세포 내 산화적스트레스의 주요 물질인 ROS의 발생을 H2DCFDA를 이용하여 측정한다. 1. ... 통해서도 apoptosis 작용 중 Vit C를 처리해주면 세포 내 변화가 덜 진행되는 것을 알 수 있었다. < Intracellular ROS detection > - ROS assay는 ... 마지막 2개의 lane을 제외한 나머지 결과는 예상했던 결과와 일치하는 그래프를 얻었다. < Griess assay > - Griess assay는 apoptosis 중 세포에서 발생하는
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
피부가 자외선에 의해 활성산소인 ROS 생성2. ROS 사이토 카인에 의해 NF-kB 생성3. MMP1 (콜라겐 분해) 증가4. 콜라겐 분해5. ... 억제 -> MMP1 억제-> 염증 관여인자- Western blot 순서1. protein Extraction: cell 또는 조직에서 단백질을 추출해내는 과정2. protein assay
1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS), FRAP assay에서 ... diphenyl-2- picrylhydrazyl(DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid(ABTS), and FRAP assay ... 또한 제브라피쉬 배아를 이용한 ROS 측정의 경우 추출 물을 처리하였을 때 감소되는 것을 확인하였다.
Planarians were homogenized and extracted to assay the contents of reactive oxygen species (ROS), lipid ... , on the other hand, glycyrrhizin-exposed planarians were significantly decreased ROS, LH levels. ... The result showed that a significantly higher level of ROS, LH indicated in planarians exposed to nicotine
, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. ... Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ... examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay
, and PC), polygonatum odoratum tea (POTA, POTB, and POTC), and jujube tea (JTA, JTB, and JTC) were assayed ... of polyphenols and flavonoids, and ability to scavenge free radicals, using two in vitro antioxidant assays ... 음료 4종(브랜드 커피 A, C 그리고 녹 차 D, 홍차 A) 시료의 농도가 높아질수록 HeLa 세포 내 활성산소종(reactive oxygen species, ROS) 생성 억제효과
Expression of pro-inflammatory cytokines was determined by enzyme-linked immunosorbent assay (ELISA). ... Down-regulation by ROS production inhibited phagocytosis and lead increased of oral pathogens-associated ... The influencing factors on phagocytosis were evaluated through the pretreatment of ROS inhibitor (N-acetyl-L-cysteine
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.
• Lipid accumulation : Oil Red O staining • ROS production: NBT assay : J. ... ROS production was assessed based on the formation of dark-blue formazan and determined by the NBT assay ... Cell viability was determined using the XTT assay.