And CEI may inhibit oocyte maturation by inhibition of oocyte denudation and extrusion of the first polar ... The objective of this study was to identify a follicular fluid ingredient inhibiting the cumulus oocyte ... Especially, fractions of RV2.15 and RV2.53 significantly inhibited COC expansion, oocyte denudation and
Oocytes from slaughterhouse ovaries were matured and fertilized using general protocol. ... in cleavage rates, but the development to blastocyst stage of oocytes exposed to sperm for 42 h was ... There was also no difference in cleavage rates, but the development to blastocyst stage of oocytes exposed
And the microinjected oocytes were incubated for 24 hours to investigate about the extrusion of second ... sperm-extract or liquid chromatographic fractions of sperm extract was microinjected into ovulated oocytes
dish에서 5~6 stage 크기의 건강한 oocytes를 분리해서 따로 모은다 7) 각 stage에 따른 oocyte의 크기와 모양 [Oocytes follicle과 collagenase ... 분 정도 실온에서 shaking 해준다 5) Oocytes가 충분히 분리 되었다고 생각되면 ND96 solution으로 4번 이상 washing 해 준다 6) Washing한 oocytes를 ... 실험제목 Xenopus laevis oocytes preparation for elecrophysiology 2.
After 24 h culture 43.8% of oocytes cultured in only TCM 199 remained at GV stage whereas 56.2% of oocytes ... Oocytes were examined for evidence of GV and GV breakdown after 24 h culture. ... Cumulus-enclosed oocytes (CEOs) were collected from follicles of 3 to 5mm diameter.
The analysis of oocyte proteins removed during enucleation is a difficult approach to the identification ... showed that both Cyclin B1 (MPF) and Erk1/2 (MAPK) were associated with the meiotic spindle of AI/TI oocytes
studies of oocyte activation and to activate reconstructed oocytes after nuclear transfer. ... in oocytes treated with 4 hr(75.5%). ... stimulation in goat oocyte activation in order to optimize the protocols.
of mature oocytes. ... of mature oocytes. 2. ... The oocytes were exposed The developmental rate of in vitro cultured oocytes recovered from ovaries collected
In Exp. 2, to evaluate of nuclear progression of immature oocytes, collected or in vitro cultured oocytes ... not affect the nuclear resumption of oocytes. ... The nuclear progression of canine oocytes checked at 24, 48 or 72 h after in vitro maturation.
In vitro matured oocytes were remove the cumulus cells using 0.2% (v/v) hyaluronidase. ... 각 발정주기별로 The aim of these experiments was to investigate in vitro nuclear maturation of canine oocyte ... Also, for nuclear maturation, in vitro culture of oocyte for 72hrs was better than 48hrs culture.
oocytes of Korean native goats. ... This study was undertaken to access the effects of collection method, room temperature at oocyte recovery ... and culture media on the oocyte quality, fertilization and cleavage rates of in vitro matured and fertilized
present study was carried out to evaluate the effect of superovulation treatments on ovarian responses, oocyte ... recovery rates and grades of collected oocytes using an ultrasound-guided transvaginal approach in Korean ... The Observation of visible follicles and collection of oocytes were performed 12 hours following the
PA and SCNT oocytes were produced by using in vitromatured pig oocytes and treated for 4 h after electric ... Post-activation treatment of PA oocytes with LA, CB, and CD did not alter embryo cleavage (85.8~88.6% ... regulators in combination with or without 6-dimethylaminopurine (DMAP) on embryonic development of pig oocytes
본 연구는 BPA 및 nicotine 첨가 농도와 배양 시간이 돼지 난자의 체외성숙에 미치는 영향을 조사하였다. BPA와 nicotine이 첨가된 TCM-199배양액에서 시간 난자를 배양했을 때 체외성 숙율을 조사하였다. BPA농도가 높을수록 체외성숙율이 유의적으로 낮..
In Experiment 1, oocytes derived from gilts were matured in three IVM media and maturation of oocytes ... Spontaneous parthenogenotes were induced by a prolonged culture of porcine oocytes in each medium. ... Oocytes matured in mSOF, NCSU-23 and TCM-199 showed no significant difference (P>0.05) in maturation
and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes ... After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm