Embryos derived from pig oocytes matured in mSOF are able to develop to blastocysts after IVF. ... An effect of media (P<0.05) was detected for mSOF on the mean number of sperm per oocyte. ... Experiment 1 evaluated the effects of two maturation media (TCM-199 vs mSOF) on maturation rate, fertilization
Maturation rates at 48h after IVM were 83.12% (mSOF), 78.03% (NCSU-23) and 83.52% (mSOF), 78.03% (NCSU ... 본 연구에서는 TCM-199, mSOF, NCSU-23 세 종류의 배지의 자연 단위 발생 유도로 형성된 돼지 배아의 초기 발육에 대한 효과를 조사하였다. ... 각 배지에서의 핵 성숙율은 mSOF군에서 83.12%, NCSU-23 군에서 78.03%, TCM-199군에서 83.52%로 세 배지의 난자 성숙율에 대한 유의적 차이는 없었다(P
the present study was to compare two different serum-free media, modified synthetic oviduct fluid (mSOF ... Hatching rate was significantly higher in control (69.0%) than other experimental groups (mSOF, 22.0% ... there was no significant difference in cleavage and blastocyst formation rates among three groups (mSOF
-mSOF : 변형된 유사 난관액 -BSA해서 용액속에 녹인 것이 BSA 용액으로 보통 단백질의 농도를 알고 싶을 때 standard 로 사용하는 것이다. ... Bovine dissection Medium, 실체현미경, 인큐베이터, FSH호르몬, LH호르몬, estradiol, Bovine Maturation Medium(TCM199), ,mSOF ... (IVF 100수정 용액 사용) (a)수정 하루 전 B.O+(수정용) Dish를 39도,5% (6)체외 발달(IVC, in vitro Culture) -mSOF(Modified Synthetic
The activated cloned embryos were cultured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for ... significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF
The developmental rates of bovine nuclear transplant embryos appeared to be highest in mSOF medium under ... 5% 0 condition, but no significant differences were found when compared with TCM199-BOEC and mSOF under
복제수정란은 0.8% BSA가 첨가된 mSOF 배양액으로 6∼7일 동안 체외 배양을 실시하였다. ... Nuclear transfer embryos were cultured in mSOF medium supplemented with 0.8% BSA 6∼7 days at 39 , 5%