In Group 3, combined treatment with ionomycin and SPP, the appearance of abnormal chromosomal tracts ... However, when ionomycin was followed immediately by DMAP (Group 2). only 20% of parthenotes were haploid
This study was to determine the effect of ionomycin and 6-dimethylaminopurine (6-DMAP) and/or elcetrical ... For the oncytes activated parthenogenetically with electrical stimulation with or with-out ionomycin ... These results indicated that the supplemental oocyte activation by ionomycin and 6-DMAP with electrical
:1ug/ml (stock conc. of PMA:1mg/ml and Ionomycin:10mg/ml) Methods Questions 1. ... into the 24 well plate) Treat the cells with or without mitogens at final conc. of PMA:10ng/ml and Ionomycin ... plate Table-top centrifuge Inverted microscope Hemocytometer CO 2 incubator Mitogen (PMA: 10 ng/ ul + ionomycine
활성화 처리는 전기자극법과 Ionomycin + 6-DMAP를 처리하여 단위발생을 유도하였다. ... 배반포기로의 발달율은 전기자극방법으로는 전혀 발달이 이루어지지 않았으나, ionomycin +6-DMAP 처리방법에서는 15.6%가 배반포로 발달하였다. ... 활성화를 유도하기 위하여 전기자극 및 ionomycin + 6-DMAP 처리를 하였을 때 분할율은 각각 64.1 및 76.5%로서 이들간에 차이는 없었다.
Activation with the ionomycin alone, ionomycin + DMAP and ionomycin + CHX resulted in cleavage rates ... in groups of ionomycin alone and of ionomycin + CHX (45.5% vs. 0 and 0%, respectively). ... Three methods for oocyte activation, including 5M ionomycin (5 min) alone, ionomycin + 1.9 mM 6-dimetylaminopurine
따라서 ionomycin을 가하면 칼슘 이온이 잘 통과하고, LPA가 제대로 작용했다면 ionomycin때와 비슷한, 혹은 그보다 못해도 칼슘이 새어나오는 패턴을 볼 수 있다. ... 결과 (Results) 그림 1 LPA 처리그림 2 ionomycin 처리 Ⅳ. ... Ionomycin은 bacterium Streptomyces conglobatus에 의해 만들어진 ionophore이다.
with 2.5 uM ionomycin for 1 or 5 min times and then cultured in 2 mM 6-DMAP for 2 or 4 hr. ... Ionomycin sequentially combined with 6-DMAP is now widely used to activate normal oocytes for analytical ... This indicated that 1 min ionomycin treatment was most suitable for activation of goat oocytes.
Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in ... In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. ... Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.
of bovine nuclear transfer embryos activated either with boar sperm cytosolic factor (SCF) or with ionomycin ... The cleavage rates of nuclear transfer embryos after 3 d of culture in the ionomycin/CHX treated group ... However, only one went to term in the ionomycin/CHX treatment while none of the pregnancies from the
Chromatin immunoprecipitation results demonstrated that PMA/ionomycin treatment induced the binding of ... When T cell activation was induced by phorbol 12-myristate 13-acetate (PMA) and ionomycin, RANKL expression ... In the presence of cyclosporin, an inhibitor of NFAT activation, this PMA/ionomycin‐induced RANKL expression
(ionomycin), DC+6-DMAP and ionomycin + 6-DMAP after in vitro maturation. ... activation, oocytes were treated with a 6-dimethylaminopurine (6-DMAP), a single DC-pulse (DC), calcium ionomycin
본 연구는 토끼난자에 있어서 배란 후 경과시간에 따른 탈핵률을 조사하고 ionomycin과 DMAP을 사용하여 활성화와 아울러 자체 탈핵을 유도하는 방법을 고안하였으며 아울러 이들의 ... on enucleation of metaphase-arrested oocytes and preactivated oocytes followed by treatment with 5 M ionomycin
The matured oocytes (n = 248) were activated with 5 ionomycin for 5 minutes followed by treatment with ... In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal
In this work we investigated comparatively the effects of enucleated oocytes treated with ionomycin and ... The enucleated oocytes were pre-activated by 5 min incubation in 5M ionomycin and 2 hours incubation ... In conclusion, it may be efficient to use the oocytes preactivated with ionomycin and 6-DMAP for the