미국가재 (Procambarus clarkii ) 수족관 개체군 및 국내 침입 자연개체군의 유전적 변이 연구

서지정보

ㆍ발행기관 : 한국환경생물학회 ㆍ수록지정보 : 환경생물 / 41권 / 3호
ㆍ저자명 : 강지현, 황정미, 권순직, 백민정, 박선재, 임창섭, 배연재

목차

1. 서 론
2. 재료 및 방법
2.1. 연구대상 지역 및 개체군
2.2. Genomic DNA 추출 및 미토콘드리아COI 유전자 염기서열 분석
2.3. 초위성체 (microsatellite) 마커 유전자 분석(genotyping)
2.4. 미토콘드리아 유전자 및 초위성체 마커 기반유전자 다양성 및 집단유전학 분석
3. 결과 및 고찰
3.1. 미토콘드리아 COI 유전자 다양성 및 단상형네트워크 (haplotype network) 분석
3.2. 초위성체 마커 기반 유전자 다양성 및집단유전학 구조 분석
적 요
사 사

영어 초록

The invasive red swamp crayfish, Procambarus clarkii, is native to southcentral United States and northeastern Mexico. Recently, it has been being spreading in the wild in South Korea. However, its primary sources, introduction routes, establishment, and expansion in South Korea remain unclear. Here, we analyzed genetic diversity and population genetic structures of its domestic natural populations during early invasion, commercial stock from local aquaria (a suspected introduction source), and original United States population using mitochondrial COI gene sequences for 267 individuals and eight microsatellite markers for 158 individuals. Natural and commercial populations of P. clarkii showed reduced genetic diversity (e.g., haplotype diversity and allelic richness). The highest genetic diversity was observed in one original source population based on both genetic markers. Despite a large number of individuals in commercial aquaria, we detected remarkably low genetic diversity and only three haplotypes among 226 individuals, suggesting an inbred population likely originating from a small founder group. Additionally, the low genetic diversity in the natural population indicates a small effective population size during early establishment of P. clarkii in South Korea. Interestingly, genetic differentiation between natural populations and the United States population was lower than that between natural populations and aquarium populations. This suggests that various genetic types from the United States likely have entered different domestic aquariums, leading to distinct natural populations through separate pathways. Results of our study will provide an insight on the level of genetic divergence and population differentiation during the initial stage of invasion of non-indigenous species into new environments.

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