Catechin이 Porphyromonas Gingivalis세균외막의 물리적 특성에 미치는 영향
(주)코리아스칼라
- 최초 등록일
- 2023.06.19
- 최종 저작일
- 2023.04
- 11페이지/
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서지정보
ㆍ발행기관 : 대한구강악안면병리학회
ㆍ수록지정보 : 대한구강악안면병리학회지 / 47권 / 2호
ㆍ저자명 : 성기수, 김태영, 주지민, 김다솔, 장혜옥
목차
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Matarials
2. Bacterial growth conditions
3. Isolation of outer membrane
4. TNBS Labelling
5. Effect of Catechin on the Structure of theIndividual Monolayers of OPGs: SelectiveQuenching of Py-3-Py
6. Determination of Annular Lipid Fluidity in OPGs
7. Determination of Protein Distribution in theOPGs Llipid Bilayer
8. Effect of Catechin on the Rotational Mobilityof Bulk OPGs
9. Effect of Catechin on the Separate Monolayersof OPGs: Selective Quenching of DPH
10. Fluorescence Measurements
Ⅲ. RESULTS
1. Effects of Catechin on the Rate and Rangeof Lateral Mobility in Bulk Bilayer OPGs
2. Effects of Catechin on the Range and Rateof Transbilayer Asymmetric Lateral Mobilityof OPGs Monolayers
3. Effects of Catechin on Annular Lipid Fluidityin the OPGs Lipid Bilayer
4. Effects of Catechin on Protein Distribution inOPGs−
5. Effects of Catechin on the Range ofRotational Mobility of Bulk Bilayer OPGs
6. Effects of Catechin on the Range ofTransbilayer Asymmetric Rotational Mobilityof OPGs Monolayers
Ⅳ. DISCUSSION
ACKNOWLEDGMENTS
영어 초록
Fluorescent probe were used to evaluate the effects of catechin on the structural parameters (annular lipid fluidity, transbilayer lateral and rotational mobility and protein clustering) of the Porphyromonas gingivalis outer membrane (OPGs). An experimental procedure was used on the basis of selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py and DPH. Catechin increased the bulk lateral and rotational mobility, annular lipid fluidity of OPGs lipid bilayers, and had greater fluidizing efficacy on the outer monolayer than the inner monolayer. It also caused membrane proteins to cluster. Based on these effects of catechin on OPGs, the antibacterial and antiviral actions of catechin can be partially explained.
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