Relationship between White Spot Symptom and Physiological Status of Two Penaeid Shrimps

서지정보

ㆍ발행기관 : 한국환경생물학회 ㆍ수록지정보 : 환경생물 / 35권 / 4호
ㆍ저자명 : Su Kyoung Kim, Myung Seok Kim, Myoung Ae Park, Su mi Kim, In Kwon Jang, Seok Ryel Kim, Miyoung Cho

목차

INTRODUCTION
MATERIALS AND METHODS
1. Morphological measurement and diseasedetection
2. RNA/DNA content and ratio
3. Trypsin analysis
RESULTS
1. Morphological parameters
2. RNA/DNA contents and ratio of F. chinensis
3. RNA/DNA contents and ratio of L. vannamei
4. Trypsin activity of F. chinensis
5. Trypsin activity of the L. vannamei
DISCUSSION
REFERENCES

영어 초록

Shrimps infected with WSSV (White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as 1.47±0.04 than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis (ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.

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