Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 43권 / 2호
ㆍ저자명 : Eun Jin Seo, Jae Kyung Park, Hoim Jeong, Jung Sook Kang, Hyung-Ryong Kim, Il Ho Jang
목차
Introduction
Materials and Methods
Cell culture
Isolation and culture of dental pulp stem cells
Organoid culture of DPCs or DPSCs
Flow cytometry analysis
Tube formation assay
Immunofluorescence staining
Statistical analysis
Results
Human dental pulp stem cells in organoid culture
Tube formation during organoid culture
Tube formation assay with organoid culture-derived cells
CD31 expression in organoid culture-derived cells
Discussion
References
영어 초록
The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.
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