자생식물 노랑꽃창포(Iris pseudoacorus L.)의 캘러스 유도 및 식물체 재분화
(주)코리아스칼라
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- 2017.01.04
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서지정보
ㆍ발행기관 : 한국자연보호학회
ㆍ수록지정보 : 한국자연보호학회지 / 6권 / 2호
ㆍ저자명 : 배기화, 유경화, 윤의수
영어 초록
This experiments were carried out to find out the effects of different explant materials, kinds and concentration of plant growth regulators on the in vitro regeneration of Iris pseudoacorus L. The effects of growth regulators on regeneration from three explant sources (root, rhizome and leaf) were more or less same. Explants produced only callus with 2,4-D treatment and other regulators had no effects. The highest frequency of callus induction (rhizome: 84%) was on MS media supplemented with 1.0 mg/L 2,4-D. For plant regeneration, when calli were transferred to the first media supplemented with 1.0 mg/L BA and 1.0 mg/L 2,4- D. Regenerated plants were successfully acclimatized in soil containing a mixture of vermiculite : perlite : leafmold (1 : 1 : 1), and their survival was high.
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