NFI-C 결손이 생쥐 치수 세포의 유전자 발현에 미치는 영향 : cDNA microarray 분석

서지정보

ㆍ발행기관 : 대한구강악안면병리학회 ㆍ수록지정보 : 대한구강악안면병리학회지 / 32권 / 3호
ㆍ저자명 : 이동설, 김성일, 한평호, 김흥중, 김현만, 고재승, 손호현, 박주철

목차

I. 서론
II. 재료 및 방법
1. 정상 생쥐와 NFI-C 결손 생쥐의 치수세포배양
2. RNA 분리 및 순도 측정
3. cDNA microarray
4. RT-PCR 분석
5. Western blot 분석
III. 실험결과
1. RNA 순도와 microarray의 scatter plot 분석
2. TGF-β/BMP 신호전달경로에 연관된 유전자군의 분석
3. Map-Kinase 신호전달경로에 연관된 유전자군의 분석
4. Cell cycle과 연관된 유전자군의 분석
IV. 총괄 및 고안
V. 참고 문헌

영어 초록

Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots. However, the mechanism by which the disruption of NFI-C gene affect the expression of other genes in dental pulp cells remains unknown. In this study, in order to understand this mechanism, the gene expression of pulp cells in NFI-C deficient mice were compared to those of wild-type mice by cDNA microarray analysis. According to the cDNA microarray profile comparison, the disruption of NFI-C gene increased the expression of TGF-β and TGF-β receptor, whereas it decreased the expression of Smad proteins. Interestingly, most of the FGF-related genes were down-regulated in pulp cells by NFI-C gene disruption. Among the cell cycle-related genes, the expression of p16 and p18 were increased by NFI-C disruption, but the expression of cy clin E1 and cy clin D1 were decreased by NFI-C disruption. These results indicate that the disturbance of NFI-C gene suppressed the proliferation of pulp cells and up-regulated the expression of TGF-β and its downstream signaling molecules during root formation, contributing to the formation of short root containing abnormal dentin.

참고 자료

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