돼지에서 HSP70 유전자형과 IVF 수정란 배 발달과의 관련성

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 20권 / 3호
ㆍ저자명 : 진현주, 김인철, 위미순, 연성흠, 김종대, 조창연, 최선호, 조상래, 손동수

한국어 초록

본 연구는 돼지에서 스트레스 관련 HSP70 유전자의 다형성과 IVF 수정란의 배발달간의 관련성을 조사하기 위하여 수행되었으며 그 결과는 다음과 같다. HSP70-K1, -K3 및 -K4의 PCR 산물로부터 SSCP 다형성은 각각 다르게 확인되었다. 체외수정란의 분할율 시험에서 HSP70 K1-AA() 및 K1-AB() 난할율은 HSP70 K1-BB()보다 유의적으로 높게 나타났다(p<0.05). 또한 HSP70 K3-AA() and K3-AB()형도

영어 초록

This study was performed to investigate the relationship between Heat shock protein 70 (HSP70) gene polymorphism and in vitro fertilization(IVF) embryo development in the pigs. The single strand conformation polymorphism(SSCP) genotypes from HSP70 K1, K3 and K4 PCR products were detected different patterns. In cleavage rate of oocyte fertilized in vitro, HSP70 K1-AA genotype() and K1-AB genotype() showed significantly higher oocyte cleavage rate than HSP70 K1-BB genotype()(p<0.05). And HSP70 K3-AA genotype () and K3-AB() also showed significantly higher oocyte cleavage rate than HSP70 K3-BB genotype()(p<0.05). The IVF embryo development of 2-cell stage according to HSP70 genotypes of sperm and pig breeds also showed a significant difference. The number of embryos developed to 2-cell stage in Landrace(28.8) and Duroc(29.8) were significantly higher than in Yorkshire(10.9)(p<0.05). And also HSP70 K4-AB genotype group(29.6) higher than HSP70 K4-AA genotype group(10.6)(p<0.05). However, the number of embryos developed to blastocyst stage did not showed significant differences among breeds as well as HSP70 genotypes. These resrults suggest that in vitro development in porcine early embryos may be affected by HSP70 genotypes and breeds.

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