소의 난포액과 호르몬이 난포란의 체외수정 및 체외발달에 미치는 영향
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 12권 / 2호
ㆍ저자명 : 최양석, 송상현, 최창용, 하란조, 강다원, 최상용, 윤창현, 박충생
ㆍ저자명 : 최양석, 송상현, 최창용, 하란조, 강다원, 최상용, 윤창현, 박충생
영어 초록
This study was carried out to determine the effect of bovine follicular fluid(bFF), hormones, and fetal bovine serum(FBS) supplemented in the medium on the in vitro fertilization and development of bovine embryos. The ovaries were obtained from a local abattoir and placed in physiological saline kept at 30~32˚C and brought to the laboratory within 3~4 hours. The oocytes and follicular fluid were collected by aspiration from visible follicles, and the oocytes of grades I on the basis of the morphology of cumulus cells attached and the homogeneity of cytoplasmic granules were selected and used for maturation. The basal media used for oocyte maturation, fertilization and embryo development in vitro were Ham' F-10, TALP and TCM-199, respectively. The hormones supplemented in maturation medium were consisted of 35 pg /ml FSH, 10 pg /ml LH and 1 pg/mi estradiol-l7. The bFF collected from 5~9 mm follicles was centrifuged, filtered and inactivated by heat-treatment at 56˚C for 30 min. FBS also was inactivated with the same method and kept at -20˚C until use. The embryos were co-cultured with the monolayer of bovine oviductal epithelial cells at 39˚C under 5% in air for 9 days. The results obtained were summarized as follows: The fertilization rate of oocytes was found 87.4% from 10% FBS and hormones treatment for IVM, and 37.1% of these TVF embryos were developed to blastocyst stage in 10% FBS groups. Compared with this control system, the fertilization rate was decreased significantly(P<0.05) in the maturation without either FBS or hormones. These IVF embryos were developed to morula stage at the similar rate, but to blastocyst at significantly(P<0.05) lower rate in the embryo culture with or without FBS supplementation. The fertilization rate(82.9%) in hormones and 10% inactivated bFF was similar with 10% FBS and hormone groups(87.4%), but decreased significantly(P<0.05) in 20 or 30% bFF (61.0 or 66.0%), respectively. In vitro developmental competence to blastocyst stage in 10% FBS and 20% inactivated bFF(37.1% and 31.4%) was higher than in 10 or 30% inactivated bFF(20.0 or 19.2%) or 10, 20 and 30% fresh bFF(19.1, 21.0 and 17.5%) The results indicated that the in vitro fertillzation and development rate of the embryos should be improved in 10% FBS or 20% inactivated culture system and 20% inactivated bFF might be available economically for bovine oocyte maturation and embryo culture instead of fetal bovine serum.참고 자료
없음"Journal of Embryo Transfer"의 다른 논문
- 과배란 방법이 Rat 수정란의 양과 질에 미치는 영향6페이지
- 토끼에서 수핵란의 세포질 활성화에 의한 제 2세대 복제수정란의 생산7페이지
- 유리화 및 완만동결법에 의한 토끼 전핵배의 동결보존 후 배발달율9페이지
- 한우 수정란의 동결보존 및 쌍자생산에 관한 연구 III. 이분 체외수정란의 배양과 동결9페이지
- Glucose가 소 초기배의 분할 및 발육에 미치는 영향9페이지
- 핵이식을 이용한 복제송아지 생산에 관한 연구 I. 세포주기, 융합배지 및 산소분압이 체외발육능에 미..9페이지
- 소 난포란의 체외수정에 있어서 정액의 처리방법이 수정 및 체외발달에 미치는 영향6페이지
- 과배란처리 한우에서 초음파 유도에 의한 난자채란에 대한 연구8페이지
- 초음파유도 난포란 채취를 위한 기본 기술의 개발 I. 초음파상에 나타난 한우 난소, 난포 및 황체의..7페이지
- 초음파유도 난포란 채취를 위한 기본 기술의 개발 II. 소의 마취방법과 채란기구의 개발8페이지