Chimeric chickens derived from qiPSCs . ... Chimeric chicken embryos derived from qiPSCs . ... from chicken embryonic stem cells (2006) Contribution of c ESCs to Stage X (EG K) chimeras Fig. 4.
Several transgenic chickens were successfully generated. ... Transgenic chickens have been spotlighted as an highly potent bioreactor for their fecundity, short ... In this study, we generated transgenic chickens exhibiting oviduct specific expression of human growth
expression of hEPO gene in the transgenic chicken. ... We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene ... The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially
We report here the generation of transgenic chickens that produce human Thrombopoietin (hTPO) using replication-defective ... chickens as bioreactors aiming mass production of commercially valuable and biological active human ... recombinant retrovirus was injected beneath the blastoderm of non-incubated chicken embryos (stage X
The results of this study may be useful in establishing stem cell-mediated transgenic chicken production ... The purpose of this study is to establish a basic culture system enabling in vitro culture of chicken ... blastodermal cells and to test the feasibility of retrovirus-mediated gene transfer to the cultured
In the study of transgenic chickens, ten of the eleven chicks hatched from 62 embryos manupulated with ... Using retrovirus vector system, the resulting recombinant hFSH gene was transferred in CHO cells and chicken ... recombinant retrovirus stock was determined to carry transgenic genes.
반대로 OT Ⅱ TCR transgenic mouse는 MHC Class Ⅱ와 마찬가지로 chicken ovalbumin에 specific한 TCR을 암호화한다. ... 그림 8 OT Ⅰ TCR tg mouse 그림 9 OT Ⅱ TCR tg mouse 다음으로, OT Ⅰ TCR transgenic mouse는 MHC Class Ⅰ와 마찬가지로 chicken ... 또한, TCR은 α chain과 β chain이 있는데, 먼저 β chain이 만들어지고 다음에 α chain이 만들어진다. β chain은 일단 CD4 CD8 double negative
of chicken b-actin promoter-driven firefly improved luciferase cDNA (β-act/luc+) and were transferred ... Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved ... Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection.
The effect of WPRE (woodchuck hepatitis virus posttrans-criptional regulatory element) sequence on transgene ... Experiments were conducted with cells derived from three different species (human, pig and chicken) and ... and CMV promoters were superior to UbC and β-actin promoters, and RSV promoter was the best one in chicken
Although avian PGCs have been used to produce transgenic birds, their characteristics largely remain ... 이후 chicken PGCs로부터 cDNA library를 구축하고 EST sequencing을 실시하였다. ... 비록 조류의 PGCs가 transgenic birds를 만드는데 사용되어 왔지만, 이것의 특징은 많이 알려지 있지 않다.
to generate transgenic pig. ... The mtDNA of chicken liver was isolated and purified by phenol and alkaline lysis extraction, and it ... After electrophoresis, DNA quantitation of each well was calculated by comparison of the band intensity
Primary culture cells (PFF, porcine fetal fibroblast; CEF, chicken embryonic fibroblast) infected with ... serious physiological disturbance problems which have been a major obstacle in successful production of transgenic ... gene under the control of the tetracycline-inducible promoters.
Primary culture cells (CEF, chicken embryonic fibroblast; PFF, porcine fetal fibroblast) infected with ... hampered successful production of transgenic animals and gene therapy. ... the recombinant FIV were cultured in the medium supplemented with or without doxycycline for 48 hours
Transformed primary culture cells (porcine fetal fibroblast, PFF, chicken embryonic fibroblast, CEF) ... serious physiological disturbance problems which have been a major obstacle in successful production of transgenic ... and phosphate, and intestinal calcium absorption.
chicken)이 보고 되었다(Shumann and Shoffner, 1986). ... 형질전환 가축(Transgenic Animal) 19세기 초 유전현상에 대한 관심이 멘델의 실험 기생충으로 부터 동물을 보호하기 위한 연구를 보고하였다. ... 것이 어렵기 때문에 닭의 생식선내에 외래 유전자를 도입시키는 수단으로 retroviral vector를 사용하는 방법이 널리 이용되었고 닭에서도 최초로 이 방법에 의해 형질전환 닭(transgenic
이산화탄소는 작은 실험동물들 (mice, rats, guinea pig, chickens, rabbits)을족해져서 저산소증을 유발시켜 동물에게 고통을 줄 수 있다. ④ 일산화탄소: ... 그 유전자를 도입한 쥐는 transgenic mouse라고 함.)를 만드는 기술은 쥐를 유전학적 방법으로 연구하는데 있어서 매우 강력한 무기이다. ... Redirect=Log&logNo=120001923399 ④ http://cafe.naver.com/medicalgenetics.cafe?
In the chicken, microinjection of DNA into newly fertilized eggs has difficulty due to the ovum coated ... Han 등 (1993)은 닭의 배아 섬유아세포(chicken embryonic fibroblast, CEF)에서 plasmid DNA(Lac Z)가 안정되게 발현된다고 보고하였고, ... During the past several years, the production of transgenic animals has been a prominent theme in poultry