Forty three COCs were in vitro matured at , 5% CO2 in air atmosphere for 48 h in a IVM medium (TCM-199 ... 62.5%이며, 핵성숙은 GV 단계(12.5%)와 M The purpose of this study was to determine the possibility of in vitro maturation
improve the developmental competence of porcine embryos, 50 nM of S1P were supplemented during in vitro maturation ... results indicate that S1P supplementation in IVM and/or IVC medium affects beneficial effect of meiotic maturation
The present study assessed the effect of FSH and LH on oocyte meiotic, cytoplasmic maturation and on ... Significant cumulus expansion and nuclear maturation was observed upon exposure to FSH alone and to the ... These data suggest that the FSH and LH enhance meiotic and cytoplasmic maturation, possibly through the
maturation (IVM) than in those without pEF. ... this study was to investigate what components of porcine epididymal fluid (pEF) influences the nuclear maturation ... When porcine GV oocytes were matured in the medium supplemented with intact pEF or pEF heated at 56'C
The supplementation of pEF during maturation of oocyte enhanced oocytes maturation in a dose-dependent ... influences the nuclear maturation of porcine germinal vesicle (GV) oocytes. ... investigate whether addition of porcine epididymal fluid (pEF) into culture medium during in vitro maturation
In this study we observed that 20% of round spermatid cultured were matured into elongating spermatid ... forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing ... embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation
In maturation rates of porcine oocytes, significant higher maturation rates was shown in the 1.0 uM group ... concentration (0, 0.1, 0.5, 1, 2 uM) of 7,8-DHF was supplemented in the porcine IVM media and then maturation
the optimal concentration of roscovitine in oocyte maturation. ... the present study, effects of concentration and time of culture in presence of roscovitine on nuclear maturation ... Nuclear maturation and morphology of the meiotic spindle and chromosomal alignment were examined to determine
was conducted to investigate the effect of hormones, protein sources and anti-oxidants on in vitro maturation ... In the maturation medium added of 100 cysteamine, the fertilization rates was 64.7 and 66.7%, respectively ... The results show that hormones, protein sources and anti-oxidants can provide a benefit for in vitro maturation
Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. ... Porcine follicular oocytes were matured in vitro for 30 to 44 hr. ... This study was conducted to investigate the effects of oocyte maturational age and activation condition
The present study was conducted to develop a simple method for porcine oocyte maturation without CO2 ... In experiment 1, we evaluated that the effect of CO2 non-supplement on porcine oocyte maturation. ... In experiment 2, we evaluated the effect of CO2 non-supplement during oocyte maturation on development
The in vitro maturation rate of vitrified-thawed canine oocytes was 30.8±3.4%. ... The in vitro maturation rate of vitrified oocytes was lower than that of the control (52.0±2.5%, p< ... The in vitro maturation rate of vitrified-thawed oocytes were significantly (p<0.05) lower than those
protein(s) during in vitro maturation than in those with anionic protein(s) (44.1% vs 20.0%). ... was to investigate what protein(s) of porcine epididymal fluid (pEF) are able to enhance the nuclear maturation ... When oocytes were cultured in the TCM 199 with fractions obtained by gel filtration, the maturation rate