transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based ... used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene ... feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene
transfer in creating transgenic pig and the other domestic transgenic animals. ... transfer in creating transgenic pig and the other domestic transgenic animals. ... Production of transgenic animals for studying specific gene has been limited due to a low efficiency,
sperm injection-mediated gene transfer (ICSI-MGT). ... 한국수정란이식학회 Journal of Embryo Transfer Hak-Jae Chung, NaRae Son, Joo-Hee Han, Chun-Gyu Park, Kyung-Woon ... The aim of present study was to determine the expression and duration of transgene transferred by intracytoplasmic
Sperm-mediated gene transfer (SMGT) can be used to transfer exogenous DNA into the oocyte at fertilization ... Transfering exogenous DNA into spermatozoa was completed within 90 min of incubation. ... The main objective of this study was to assess efficiency of transferring mitochondrial DNA (mtDNA) fragment
abundance of Bax, Bcl2L1, VEGF and FGFR2 in in vitro fertilized (IVF), parthenogenetic (PAT) and nuclear transfer ... The present study observed the abnormal gene expressions in NT embryos at various developmental stages ... set of 10 embryos at the 4-cell to blastocyst stages were used to extract total RNA for analyzing the gene
nuclear transfer. ... The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after ... the results, the isolated cells with laminin matrix selection strongly expressed the GFRα-1 and PLZF genes
transfer (mNT) embryos produced under different osmolarity culture conditions. ... This study was conducted to investigate the development and gene expression in miniature pig nuclear ... Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts
This study investigated the developmental ability and gene expression of somatic cell nuclear transfer ... 한국수정란이식학회 Journal of Embryo Transfer Park, Mi-Rung, Hwang, In-Sun, Shim, Joo-Hyun, Moon, Hyo-Jin, Kim ... However, imprinting genes were expressed aberrantly in mNT compared to pNT blastocysts.
Several cloned animals have been produced using somatic cell nuclear transfer (SCNT) and have interested ... 한국수정란이식학회 Journal of Embryo Transfer Jang, Goo, Ko, Kyeong-Hee, Jeon, Hyun-Yong, Lee, Byeong-Chun ... The purpose of this study was to analyze gene expression pattern in in vitro fertilized (IVF) or SCNT
실험실에서 형질전환될 수 있는 세균들은 자연환경 조건에서도 형질전환 능력이 발달하는 것으로 알려져 있다. 따라서, 환경 내에서 형질전환 능력이 있는 세균의 존재는 확실한 것으로 여겨진다. DNA는 무기물에 부착된 상태에서는 핵산분해효소에 의한 분해로부터 보호되는 것으로..
The possible gene transfer from transgenic chili pepper to soil bacteria in rhizosphere was evaluated ... transfer from transgenic chili pepper to culturable rhizosphere soil microorganisms was not occurred ... The results of the PCR targeting NPTII gene indicated that the transgene was present in the rhizosphere
Gene Transfer to Mammalian cells ★실험목표★ plasmid DNA를 cell line(BJAB)내로 유입시켜 세포 내에서 발현되도록 하는 것이 transfection의 ... Acetylation 된 정도를 정량화 해서 CAT 유전자의 발현 정도를 확인한다. - Luciferase : reporter gene으로서 반딧불이의 유전자인 luciferase를 ... 원리 특정 유전자의 promoter 혹은 regulatory element를 reporter gene의 5' region (upstream)에 cloning하여 reporter vector를
< 현대생물학 실험 보고서 > Gene Transfer to Mammalian Cells - transfection & transduction - 자연과학부 기초과학계 2001-11084 ... 그런데 이번 실험의 경우, packaging signal이 luciferase gene 앞에 있기 때문에, luciferase gene을 포함하고 있는 LTR(long terminal ... 배지에는 G418이 처리되어 있기 때문에 neo gene이 있는 cell만 즉, transduction이 일어난 cell만 살 수 있다.
전기영동이 끝난 후 젤을 Transfer buffer에 넣는다. Transfer는 단백질을 나일론 막으로 옮기는 작업이다. ... (단백질이 아래쪽으로 이동할 것을 가정하였을 때) 판 위에 Transfer buffer를 적신다. 100V에서 90분간 transfer한다. ... Alpha Tubulin은 Housekeeping Gene으로 항상 동일한 발현양을 나타내야 한다.