Oocyte is the central factor in the bi-directional communication axis in the ovarian follicles. ... In conclusion, by regulating functions of granulosa/cumulus cells, oocyte has the potential to enhance
We also found ring shaped signal which represent autophagosome near oocyte membrane. ... Oocyte maturation is dynamic process, in which various proteins should be synthesized and degraded. ... previous study, we reported the loci of autophagosome and dynamics of autophagic activity in porcine oocytes
Pig oocytes have also shown ER stress mostly in metaphase II stage. ... ER stress in oocytes may hinder the in vitro production of pig embryos. ... Glutathione (GSH) content is an indicator of cytoplasmic maturation of oocytes.
via elevated GSH and reduced ROS levels in oocytes. ... Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. ... Many studies have been reported that intracellular reactive oxygen species (ROS) in mammalian oocytes
Brilliant cresyl blue test has been used to select the good quality of oocyte. ... Cumulus oocyte complexes (COCs) have been exposed in 75 M of Rosco for 22 and 44 h. ... was to explore the effects of Roscovitine (Rosco) prior to in vitro maturation (IVM) of immature pig oocyte
(DO) and cumulus cell-oocyte-complex (COC) during IVM process (22 and 44 h), respectively. ... However, the role of gangliosides GD1a for EGFR-related signaling pathways in porcine oocyte is not yet ... important factor for meiotic and cytoplasmic maturation during in vitro maturation (IVM) of mammalian oocytes
Immature pig oocytes were matured for 44 h and then oocytes reached metaphase II (MII) stage were electrically ... was designed to determine the effect of monosodium glutamate (MSG) on in vitro maturation (IVM) of oocytes ... -87.1%), intra-oocyte glutathione content, cumulus expansion, and cleavage (91.4-93.4%) of PA embryos
) (84.5±14.2%) than that of vitrified oocytes. ... Cryopreservation of oocytes by vitrification technique may contribute a lot in the field of reproductive ... present study were to evaluate the effectiveness of two cryo-devices for vitrification of immature oocytes
Intracellular GSH levels in oocyte treated with 5μM of FA significantly increased (P < 0.05), and ... Oocytes treated with FA exhibited significantly higher cleavage rates (79.01% vs 89.19%, 92.20%, 90.89% ... Oocytes treated with 10μM showed significantly higher blastocyst formation rates (28.3% vs 40.3%, respectively
compared with SIR oocytes. ... In addition, IVM of oocytes in medium with reduced NaCl increases the proportion of SSP oocytes in pigs ... We found that SSP oocytes showed improved developmental competence after PA and SCNT.
Oocytes matured with 50 uM of ALA during IVM displayed significantly higher cleavage rates (67.8% vs. ... To examine the effect of ALA on the in vitro maturation (IVM) of porcine oocytes, we investigated intracellular ... Intracellular GSH levels in oocytes treated with 50uM ALA increased significantly (P < 0.05) and exhibited
The purpose of this study is to improve the maturation of oocytes derived from follicles ≤ 3 mm in diameter ... In cumulus cells, PACAP receptors, ADCYAP1R1 and VIPR1 were detected but were not detected in oocytes ... In conclusion, these results indicated that even though the oocytes were derived from SF, pre-IVM application
rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats ... The mean number of oocytes recovered per ovary was . ... No pronucleus formation was observed in control oocytes.
maturation of canine immature oocytes in vitro. ... according to oocyte diameter and different culture environments. ... The present study was performed to determine the ability of canine oocytes to achieve nuclear maturation