Fusion, first cleavage and blastocyst developmental rate was not different between fetalfibroblast and ... fibroblast and adult skin fibroblast as donor nuclei. ... fibroblast cell of male origin after 65 days embryo transfer.
performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts ... And the fibroblasts derived from Han Woo can be reprogrammed by nuclear transplantation and develop subsequently
Porcine fetal fibroblasts were isolated from 33-day-old fetuses. ... In this study, we established an efficient culture method of porcine fetal fibroblasts. ... The proliferation of porcine fetal fibroblasts was analyzed by different serum types and culture media
Transgene construct including hCD59 and ILI-18BP was introduced into miniature pig fetal fibroblasts. ... After antibiotic selection of double transgenic fibroblasts, integration of the transgene was screened ... was designed to produce nuclear transfer embryos, prior to the production of transgenic pigs, using fibroblasts
and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. ... fibroblasts in culture. ... These cells were grown under four different culture conditions: no serum (negative control), 10% fetal
The development potential of bovine somatic cells was evaluated using nuclear transfer. A single donor cell derived from fetus of HanWoo(Korean Nativ..
The fetalfibroblast cells (KbFF) were isolated from a 45-day male fetus. ... fibroblasts. ... nuclear transfer, enucleated bovine oocytes were transferred with hFSH transfected or nontransfected fetal
fibroblast cells. ... the effects of catalase and -mercaptoethanol (ME) on the establishment of clonal lines from porcine fetal ... Fibroblasts derived from a pig fetus (Day 50) were passaged two times before use.
그 이후 fetal monkey fibroblast와 enucleated oocyte간의 fusion이 일어나는 것을 확인한다. ... 결국 연구진들은 fetal monkey fibroblasts를 이용한 SCNT를 활용해 두 마리의 건강한 cynomogus monkeys clones를 탄생시켰다. ... 추가적으로 non-human primate의 cloning을 위한 source로서 fetal cell nuclei가 cumulus cell보다 더 효과적이라는 점과 Kdm4d와 TSA를
Abstract이번 실험에서는 AsPC 1 Human Dermal Fibroblast 를 Fetal brovine serum(FBS) 와 culture media(DMEM) 을 이용해 ... 진행 하고 3 일전 접종한 세포의 상태 (passage 전 ), passage 직후 , passage 한 후 4 일 뒤의 세포의 상태와 모습을 관찰 했 으며 Human Dermal Fibroblast
예를 들어, 인간 섬유아세포(human fibroblast)인 MRC5는 약 30세대 분열 후 노화되어 더 이상 분열되지 않는다. ... 이때 얻어지는 2차 배양세포(secondary cell line)는 소태아 혈청(FBS, fetal bovine serum)이 포함된 배지(media)에서 배양하며 대부분은 부착의존성 ... 배양에서 사용되는 인체세포의 형태는 세 가지로 비부착의존성 세포인 림프구(lymphocyte), 부착의존성 세포인 상피세포(epithelial), 그리고 섬유아세포(fibroblast